Kilbane 2009 R&D Summary

1. Energy Biotechnology John J. Kilbane II Illinois Institute of Technology [email_address]

12. Anaerobic digestion of water hyacinth and kelp to make methane.

13. GTI pioneered the development of two-phase anaerobic digestion of biomass to make methane. The reactor pictured here is at the Woodridge, IL wastewater treatment plant.

16. Microbial Corrosion A faster, more accurate, and less expensive method to detect microorganisms associated with corrosion was developed from concept through commercialization .

18. Genetic Techniques Were Used to Characterize the Microbial Ecology of Gas Pipelines

21. New Techniques to Investigate Microbial Diversity Were Developed

22. Demonstration of Flow Cytometry to Characterize Untreated and Stained Cell Populations

23. Analysis of 16S RNA Genes by DGGE Reveals that FACS Allows the Fractionation of Complex Microbial Communities and the Cultivation of Species not Detectable Using an Unfractionated Inoculum 4AF 7.1AF 7.2AF 8AF 9AF 10AF 1 2 3 4 5 6 7 8 9 10 11 1 2 3 4 5 6 7 8 9 10 1 2 3 4 5 6 7 8

30. Purification of DszA and DszC proteins from Rhodococcus using reverse phase and ion exchange chromatography.

32. Pioneering Research of Gene Expression in Rhodococcus RT-qPCR Northern Blot to Detect mRNA

33. Robotic platform used in screening of enzymatic mutants.

34. Typical results of mutant screening using different substrates.

36. Kinetic analysis of DszA enzymatic activity of wild type and two mutant derivatives.

39. Specific desulfurization activity of dsz genes in two species  , M. phlei GTIS10 ;  , R. erythropolis IGTS8.

41. Gene Expression in T. thermophilus

42. Genetic Manipulation of T. thermophilus

45. Thanks! John Kilbane Illinois Institute of Technology [email_address]