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En vedette

Tumors are becoming recognized as genetically heterogeneous masses of cells with different clonal histories. Identifying the mutations present in these heterogeneous masses can lead to important insights into the future behavior of the tumor and possible intervention mechanisms. However, the rarity of pathogenic mutations in small subsets of cells can make identification of such alleles difficult. In this study, we demonstrate a complete workflow that facilitates the identification of rare and novel alleles from FFPE tumor sections. We collected small regions with different cellular morphologies from lung tumor samples using laser capture microdissection, extracted both DNA and RNA from these regions, and characterized mutations present and transcript abundances by using Ion AmpliSeq™ targeted sequencing. We show that LCM facilitates the detection of alleles that are not detectable in macrodissected tissue scrapes. We also show that different regions of a tumor have very different patterns of alleles detectable and have a great deal of genetic diversity. Finally, we show that RNA expression patterns are also clearly different in the different regions. Interestingly, dissected regions with similar gross tissue morphologies display differences in alleles present and RNA expression patterns. These results suggest how we may in the future use this method to analyze mutations present in a tumor is to microdissect different subregions of the tumor, and using Ion AmpliSeq™ panels to identify the alleles present in those subregions.

Identification of Rare and Novel Alleles in FFPE Tumor Samples | ESHG 2015 Po...

Thermo Fisher Scientific

Hadjer

noorhadjer

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Cristina Anjos Silva

Cupcakealicious

mkohlerwsm

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Imesha Ruwan Pathirana

This is a Project Report which was prepared for an Assignment at National Institute of Business Management. This includes a background study and a macro environmental Analysis of Sri Lankan Tourism Industry. This is done as the Marketing Management module assinment of BSc. In Business Management (Special) Degree of National Institute of Business Management. Group Members: P.D.N.J. Anjana D.M.M.S Jayakody K.S.M.Perera N.G.Pathmanathan T. Kurubaran T. Subramaniam

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Navaka Navaratne

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Identification of Rare and Novel Alleles in FFPE Tumor Samples | ESHG 2015 Po...

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