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1. Question set for ICAR-JRF/SRF/NET
Major subject: - Plant biotechnology
Major asked question list for ICAR-JRF/SRF/NET

2. 1. The most commonly used medium for tissue culture
(a) MS medium (b) B6 medium
(c) N & N medium (d) LS medium
2. A natural genetic engineer in genetics is
(a) Agrobacterium (b) Fusarium
(c) pseudomonas (d) trichoderma
3. The semi-conservative mechanism of replication was demonstrated by
(a) Meselson and stahl (b) hershey and chase
(c) Watson and crick (d) Carlson and weber
4. protoplast fusion method includes
(a) polyethylene glycol method
(b) electric fusion
(c) Treating with sodium nitrarte
(d) All of above
5. molecular chapernoes is associated with
(a) protein synthesis
(b) protein folding
(c) lipid synthesis
(d) protein denaturation
6. hybridoma technology is used for the production of
(a) polyclonal antibodies
(b) monoclonal antibodies
(c) chimeric antibodies
(d) somaclonal antibodies
7. which of the following DNA structure forms right hand helix. ?
(a) DNA B (b) DNA C
(c) DNA Z (d) DNA E
8. The genes is responsible for the fixation of nitrogen is
(a) nif genes (b) His genes
(c) gif genes (d) none of above
9. Actinomycin-D, rifampicin and 5-bromouracil are the inhibitors to the
synthesis of
(a) mRNA (b)tRNA (c)rRNA (d) None of above
10. The phenomenon in which highly concentrated aqueous solutions of cryo-
protective agents become so vicious at sufficiently low temperatures that
they solidify into a meta-stable glass state without ice crystal formation at
practicle cooling rates is called

3. (a) transpiration (b) transgenic silencing
(c) vitrification (d) transversion
11. In lac Operon, allolactose is act as
(a) gratituous inducer (b) suppressor
(c) substrate (d) inducer
12. Termination codon for protein synthesis are
(a) UGA,UAA,UAG
(b) AUU, AUG,GUG
(C) AAA,UUU,UAG
(D) UAU, UGA,GUG
13. Smooth ER is associated with
(a) lipid synthesis
(b) protein synthesis
(c) both
(d) none of above
14. An anticodon of tRNA can recognize more than one codon mRNA, it is
(a) wobble hypothesis
(b) mendle hypothesis
(c) gene flow hypothesis
(d) Template hypothesis
15. The nitrogenous base present in lecithin is
(a) choline (b)inositol
(c) serine (d) ethanolamine
16. Which is the precursor of vitamin A
(a) Beta carotene (b) serine
(c) tryphtophan (d) tyrosine
17. Mutant variety of rice
(a) jagganth (b) PUSA basmati
(b) karnal local (d) jasmine rice
18. CO2 content in the atmosphere is
(a)0.003% (b) 0.3%
(c)0.03% (d) 3.0%
19. Potential biofertilizer for rice
(a) Sesbania (b) azolla
(c) Cyanobacteria (d) blue green algae
20. Pusa chamtkar is variety of
(a) Mango (b) green gram

4. (c) Chickpea (d) black gram
21. Urea contains ...........% nitrogen
(a) 96% (b) 56%
(c)46% (d)26%
22. Total numbers of KVK in india till 2016 is
(a)551 (b)642
(c)532 (d)670
23. A hormone Is used as a herbicide.
(a)auxin (b) etlylene
(c)zibbrellin (d)2-4D
24. Red color of tomato is due to
(a) carotene (b) phenol
(c) lycopine (d) xanthophyll
25. Rice dwarf variety which was first developed in world
(a)TN-1(taiwan)(b) TN-2
(c)TN-3 (d) TN-4
26. which The most critical stage during the growth of wheat for irrigation.?
Or transition zone between root and shoot is ....
(a) Flowering stage (b) grain formation stage
(c) CRI(Crown root initiation) stage (d) Late tillering stage.
27. Sugarcane is .......... plant?
(a)C3 plant (b) C4 plant
(c) CAM plant (d) none of above
28. causal agent of mad cow disease is
(a) virus (b) bacteria
(c)viriods (d) prions
29. R.B.I. was established in which year
(a)1930 (b) 1935
(c) 1945 (d) 1948
30. Which crop is maximum oil seed producer in India?
(a) sunflower (b) canola
(c) soybean (d) ground nut
31 QPM (quality protein maize) was developed by which gene?
(a) Flory gene (b) Opaque-2
(c) P cluster family (d) zain-2
32. Which is tag color of certified seed?
(a) Blue (b) green

5. (c) yellow (d) orange
33. How many acres are in one hectare?
(a)2.47 acre (b)1.50 acre
(c)1.47 acre (d) 1.27 acre
34. Charcoal rot is due to which organism ?
(a) mactophomina spp. (b) Helmonthosporium
(c) phytopthora infestance (d) E. coli
35. In which plants stomata open during night?
(a )C3 plants (b)C4plants
(c)CAM plants (d) none of above
36. Precursors of Ethylene is.
(a)Methionine (b)proline
(c)serine (d) asparagines
37. Diameter of silt particle is
(a) 0.02-0.002 mm(b)0.2-0.02 mm
(c)> 2.0 (d) 2.0-2.2 mm
38. PCR was invented in the 1984 by
(a)kornberg (b) kary mullis
(c)sanger (d) gillbert
39. During nitrogen fixation, the enzyme nitrogenase catalyze the reaction is
require approximately
(a)12 ATP (b) 18 ATP
(c) 25 ATP (d) 29 ATP
40. Most deficient micronutrient in Indian soil is
(a) Zn (b) B (c) Cu (d) Mn
41. Experimental design suitable for field having one directional fertility
gradient is
(a) LSD (b) CRD (c) RBD (d) SPD
42. In ascent of sap, the water moves up in plants through
(a)Phloem (b) endodermis (c) cambium (d) xylem
43. in plants, maximum photosynthesis occurs under solar waves of
(a) Blue wavelength (b) red wavelength
(c) Green wavelength (d) yellow wavelength
44. which is not measure of dispersion ?
(a) mode (b) range
(c) S.D. (d) mean deviation
45. which are the measure of central tendency ?

6. (a) Mean (b) median
(c) mode (d) all of above
46. SRI technique of paddy has been developed at
(a) Japan (b) china
(c) Madagascar (d) korea
47. First product of C3 pathway is
(a) PGA (b) PEP
(c) Oxaloacetic acid (d) tricarboxylic acid
48. First product of C4 pathway is
(a) phosphoglyceraldehyde (b) Oxaloacetic acid
(c) melic acid (d) PEP
49. first KVK of india was established at
(a)hyderabad (b) nagpur
(c) Chennai (d) pondicherry
50. Study of soil in relation to plant is called
(a) pedology (b) edaphology
(c) Climatology (d) entomology