1.
Feb. 2023
The Israeli BioFoundry
Releasing the Genie Out of the Bottle

2.
Synthetic Biology: Taking biology into engineering mode

3.
The Needs
Those who want to venture into the field
of SynBio currently can’t, due to:
• Lack of physical infrastructure and professional equipment
• High equipment and construction costs
• Lack of professional support and guidance
• Lack of scientific resources and ecosystem
• Lack of a customer-oriented service center

4.
A Unique Venture with Complementary Expertise
Academic partners with a
commercialization mindset
Established & experienced, highly
regulated service company
Michael
Eisenberg
Israel’s leader in venture capital and
high-growth startups

5.
Our Vision
Engineering life for the
benefit of humanity
Alagene is a home for innovation and R&D center in SynBio, serving as a
one-stop—shop for knowledge, methods, and instrumentation to enable our customers in
Israel and worldwide to get to their proof of concept.

6.
An End-To-End Solution
Infrastructure supporting gene & host
optimization in microbial systems
Computational
Design
Construct & Host
Engineering
Optimization by
Automation &
Analytics
Precision Fermentation,
Pre-Production & POC

7.
Enabler of Two Major Implementations
Zhang et al.,
2022
 56 genetic edits
 34 heterologous genes
Production of Anti-Cancerous
2ndary Plant Metabolite in
Yeast
Scalable platform
 >3,000 natural alkaloids
 Infinite number analogues
Market Sectors
 Therapeutics
 Agritech
 Cleantech &
safety
 Security
 Materials
 Others
Biomanufacturing – Going Wild! Direct Microbial Application – Sky’s the Limit!

8.
Host
Engineering
Random
Mutagenesis
Cell Sorting
(Selection)
Cell Screening
Mating &
Sporulation
Precision
Fermentation
Development of a Dairy Milk Producing
System
Rational
Design
• Induction
• PTM
Directed Evo.
• Copy number
3 iterations
Objective: fermentation yield of ≥1 g/L
Alagene designed the following SoW
Assay setup &
calibration
Assay setup &
calibration in
collaboration
with
specialized
academic group
Assay setup &
calibration
Assay setup &
calibration
Assay setup &
calibration
Genetic Optimization Process Optimization

9.
Gene Optimization
& Host Engineering
Protein
Purification
& Preparation
Cell Screening &
Analysis
Bioactivity
Precision
Fermentation
Genetic Optimizatiom Process Optimization
 Codon
optimization
 Expression
module
 Auxiliary
mutations
 Assay setup &
calibration
 Screening by
yield  Process setup &
calibration
 QC
Validation
 Assay setup &
calibration
SynBio project cycle

10.
A few project examples:
 Bio production of egg protein
 Computational modeling of full metabolic
network in Algae
 Bio production of rare sugars with a low
glycemic index
 Computational prediction for introducing
new genes into microbes
 Precision fermentation for yeast producing
dairy proteins
 Bio production of psychoactive /
cannabinoid molecule in yeast
 Bio production of growth factors for the
cultured meat industry
 Developing new inhouse tools to support
Industry: Academia:

11.
Thank You