Keane, Robert

Innate and adaptive immune responses Clinical Immunology: Principles and Practice. Chapter 3: Innate Immunity. Rich et al.

Martinon et al., 2009, Ann. Rev. Immunol. 27:229-265.

Duprez et al. Microbes & Infect. 11:1050

What Comprises the Innate Immune System in the CNS? Which CNS Cells Harbor Inflammasomes? What is the Regulation of the Innate Immune System After CNS Injury?

Primary vs . secondary injury

SCI induces increased expression and processing of IL-1  and IL-18

SCI induces increased expression of Caspase-1, -11 and ASC

de Rivero Vaccari JP, Lotocki G, Marcillo AE, Dietrich WD, Keane RW. (2008). J Neurosci. 28: 3404-3414

Association of NLRP1 Proteins in Neurons

Confocal images of spinal cord sections demonstrating anti-ASC antibody penetration into spinal cord neurons de Rivero Vaccari JP, Lotocki G, Marcillo AE, Dietrich WD, Keane RW. (2008). J Neurosci. 28: 3404-3414

ASC neutralization blocks NALP1 inflammasome activity de Rivero Vaccari JP, Lotocki G, Marcillo AE, Dietrich WD, Keane RW. (2008). J Neurosci. 28: 3404-3414

ASC neutralization improves histopathological outcome and decreases spinal cord lesion volume de Rivero Vaccari JP, Lotocki G, Marcillo AE, Dietrich WD, Keane RW. (2008). J Neurosci. 28: 3404-3414

de Rivero Vaccari JP, Lotocki G, Marcillo AE, Dietrich WD, Keane RW. (2008). J Neurosci. 28: 3404-3414 Anti-ASC treatment improves functional outcomes after SCI

ASC neutralization decreases NLRP1 inflammasome activity

ASC neutralization improves histopathological outcomes after TBI

Conclusions ,[object Object],[object Object],[object Object]

High Extracellular K+ Activates Caspase-1 in Neuronal and Astrocytic Cultures

Extracellular ATP Activates Caspase-1 in Human Astrocytic Cultures

ATP-Induced Caspase-1 Activation is Blocked by BBG and Probenecid

Pyroptosis is induced by the inflammasome, a multi-protein complex that stimulates caspase-1 cleavage ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

Poly(dA:dT) Induces a Dose-Dependent Innate Immune Response in Cortical Neurons ,[object Object],[object Object]

Poly(dA:dT) Increases Expression of AIM2 Inflammasome Components ,[object Object],[object Object]

Poly(dA:dT) Induces Assembly of the Pyroptosome and Subsequent Cell Death Cell Viability Assay Oligomerization of ASC

Model of Neuronal Pyroptosis after CND Injury

Summary ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

Dr. Juan Pablo de Rivero Vaccari Dr. Gerhard Dahl Dr. W. Dalton Dietrich Acknowledgments Julia Minkiewicz Stephanie Bacik

Keane, Robert

Recommandé

Recommandé

Contenu connexe

Tendances

Tendances (20)

En vedette

En vedette (16)

Similaire à Keane, Robert

Similaire à Keane, Robert (20)

Plus de National Neurotrauma Symposium

Plus de National Neurotrauma Symposium (20)

Dernier

Dernier (20)

Keane, Robert