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Microbial	
  Life	
  in	
  Your	
  Home	
  
	
  
Project	
  Directors:	
  
	
  
Noah	
  Fierer	
  (Assoc.	
  Professor,	
  Univ.	
  of	
  Colorado)	
  
	
  
Rob	
  Dunn	
  (Assoc.	
  Professor,	
  NC	
  State	
  Univ.)	
  
	
  
	
  
Project	
  Personnel:	
  
	
  
Holly	
  Menninger	
  (Director	
  of	
  Public	
  Science,	
  	
  
       	
      	
   	
     	
   	
  NC	
  State	
  Univ.)	
  
	
  
Jon	
  Leff	
  (Research	
  Assoc.,	
  Univ.	
  of	
  Colorado)	
  
	
  
Gilberto	
  Flores	
  (Postdoc,	
  Univ.	
  of	
  Colorado)	
  
Project	
  Goals:	
  
	
  
1.  Explore	
  broad	
  paKerns	
  governing	
  the	
  distribuLon	
  of	
  
     bacteria	
  and	
  fungi	
  in	
  houses	
  across	
  the	
  U.S.	
  

2.  Generate	
  interest	
  in	
  the	
  unexplored	
  microbial	
  diversity	
  
    found	
  all	
  around	
  us	
  

3.  Engage	
  ‘ciLzen	
  scienLsts’	
  in	
  the	
  scienLfic	
  process	
  of	
  
    exploring	
  microbial	
  ecology	
  
Skin-­‐assoc.	
  taxa	
  




                                                   Fecal-­‐assoc.	
  taxa	
  




                                                    Soil-­‐assoc.	
  taxa	
  




Flores	
  et	
  al.	
  PLoS	
  One.	
  2011.	
  
Microbial	
  biogeography	
  of	
  the	
  kitchen	
  




PCo1	
  scores	
  –	
  kitchen	
  #1	
  
Propionibacterium	
  (#69)	
                            Corynebacterium	
  (#6)	
  




Pseudomonas	
  (#3)	
                    Erwinia	
  (#12)	
  
Salmonella	
  (#409)	
  


Campylobacter	
  (#302)	
  




                  Listeria	
  (#1044)	
  
How	
  does	
  microbial	
  diversity	
  outside	
  homes	
  relate	
  to	
  
that	
  found	
  inside	
  homes?	
  
	
  
	
  
How	
  does	
  x	
  influence	
  house-­‐associated	
  bacterial	
  and	
  
fungal	
  communiHes	
  across	
  the	
  U.S.?	
  
	
  

                  x	
  =	
      	
  	
  
                        	
  presence	
  of	
  pets	
  in	
  the	
  home	
  
                        	
  climate	
  
                        	
  surrounding	
  land-­‐use	
  type	
  (e.g.	
  urban	
  vs.	
  rural)	
  
                        	
  house	
  occupancy	
  
                        	
  vegetarian/non-­‐vegetarian	
  
                        	
  house	
  type	
  (e.g.	
  free-­‐standing	
  vs.	
  apartment)	
  
                        	
  house	
  characterisLcs	
  (e.g.	
  carpeted	
  vs.	
  bare	
  floors)	
  
       	
  	
  
       	
  	
  
	
  
	
  
	
  
hKp://www.yourwildlife.org/	
  
Ongoing	
  work:	
  
	
  
     -­‐	
  	
  Sequencing	
  and	
  analysis	
  of	
  samples	
  collected	
  from	
  20	
  homes	
  in	
  
                North	
  Carolina	
  
     	
  
     -­‐  Environmental	
  scanning	
  electron	
  microscopy	
  of	
  key	
  house-­‐
                associated	
  microbial	
  habitats	
  

     -­‐  Volunteers	
  recruited	
  from	
  >6,000	
  houses	
  across	
  the	
  U.S.	
  

Planned	
  work	
  (next	
  6	
  months):	
  
	
  
     -­‐  Analyze	
  samples	
  from	
  >1000	
  houses	
  
     -­‐  IdenLfy	
  and	
  establish	
  “super-­‐sampler”	
  homes	
  for	
  longer-­‐term	
  
          sampling	
  
     -­‐  Design	
  experiments	
  and	
  more	
  directed	
  studies	
  to	
  build	
  on	
  
          research	
  findings	
  
Pilot	
  Study	
  (20	
  homes	
  near	
  Raleigh,	
  NC)	
  
Toilet	
  Seat	
     Pillow	
  case	
  
              TV	
  Screen	
  




                                                                                       Refrigerator	
  shelf	
  
       Door	
  knob	
  



                                                                                                             Kitchen	
  counter	
  


                                                                                        CuWng	
  Board	
  
Door	
  Sill	
  (outer)	
  


                                 Door	
  Sill	
  (inner)	
  
 
                          	
  
6,127	
  households	
  signed	
  up	
  to	
  date	
  	
  
           (as	
  of	
  12-­‐28-­‐2011)	
  
                          	
  
                          	
  

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Citizen micro fierer_1-2012

  • 1. Microbial  Life  in  Your  Home     Project  Directors:     Noah  Fierer  (Assoc.  Professor,  Univ.  of  Colorado)     Rob  Dunn  (Assoc.  Professor,  NC  State  Univ.)       Project  Personnel:     Holly  Menninger  (Director  of  Public  Science,              NC  State  Univ.)     Jon  Leff  (Research  Assoc.,  Univ.  of  Colorado)     Gilberto  Flores  (Postdoc,  Univ.  of  Colorado)  
  • 2. Project  Goals:     1.  Explore  broad  paKerns  governing  the  distribuLon  of   bacteria  and  fungi  in  houses  across  the  U.S.   2.  Generate  interest  in  the  unexplored  microbial  diversity   found  all  around  us   3.  Engage  ‘ciLzen  scienLsts’  in  the  scienLfic  process  of   exploring  microbial  ecology  
  • 3. Skin-­‐assoc.  taxa   Fecal-­‐assoc.  taxa   Soil-­‐assoc.  taxa   Flores  et  al.  PLoS  One.  2011.  
  • 4. Microbial  biogeography  of  the  kitchen   PCo1  scores  –  kitchen  #1  
  • 5. Propionibacterium  (#69)   Corynebacterium  (#6)   Pseudomonas  (#3)   Erwinia  (#12)  
  • 6. Salmonella  (#409)   Campylobacter  (#302)   Listeria  (#1044)  
  • 7. How  does  microbial  diversity  outside  homes  relate  to   that  found  inside  homes?       How  does  x  influence  house-­‐associated  bacterial  and   fungal  communiHes  across  the  U.S.?     x  =        presence  of  pets  in  the  home    climate    surrounding  land-­‐use  type  (e.g.  urban  vs.  rural)    house  occupancy    vegetarian/non-­‐vegetarian    house  type  (e.g.  free-­‐standing  vs.  apartment)    house  characterisLcs  (e.g.  carpeted  vs.  bare  floors)                
  • 9. Ongoing  work:     -­‐    Sequencing  and  analysis  of  samples  collected  from  20  homes  in   North  Carolina     -­‐  Environmental  scanning  electron  microscopy  of  key  house-­‐ associated  microbial  habitats   -­‐  Volunteers  recruited  from  >6,000  houses  across  the  U.S.   Planned  work  (next  6  months):     -­‐  Analyze  samples  from  >1000  houses   -­‐  IdenLfy  and  establish  “super-­‐sampler”  homes  for  longer-­‐term   sampling   -­‐  Design  experiments  and  more  directed  studies  to  build  on   research  findings  
  • 10. Pilot  Study  (20  homes  near  Raleigh,  NC)  
  • 11.
  • 12. Toilet  Seat   Pillow  case   TV  Screen   Refrigerator  shelf   Door  knob   Kitchen  counter   CuWng  Board   Door  Sill  (outer)   Door  Sill  (inner)  
  • 13.     6,127  households  signed  up  to  date     (as  of  12-­‐28-­‐2011)