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THE REMOVAL OF HEAVY METALS BY
MICROORGANISMS: BIOREMEDIATION AT
             WORK
              A Review Paper submitted by
                MARGARET DE GUZMAN
           As fulfillment for the requirement in
                        EDSC 350
         (Advance Topics in Biology for Teachers)
INDUSTRIALIZATION
WASTEWATER TREATMENT




A polluted creek covered with trash in Manila,
Philippines on 01 March 2009. The Department of
Environment and Natural Resources reported in 2008
that the Philippines hosts 50 major polluted rivers,
with a majority of pollutants coming from domestic
waste
http://www.csuwai.ws/heavymetal/images/
metal_contamination3.jpg
SOURCES OF HEAVY METALS IN OUR HOMES




                       HEAVY METAL SOURCES & HOW THEY AFFECT US
HEAVY METALS
• chemical elements with a specific
  gravity that is at least 5 times the
  specific gravity of water. The
  specific gravity of water is 1 at
  4°C (39°F).
• Some well-known toxic metallic
  elements with a specific gravity
  that is 5 or more times that of
  water are arsenic, 5.7; cadmium,
  8.65; iron, 7.9; lead, 11.34; and
  mercury, 13.546 (Lide 1992).
The main threats to human health from
heavy metals are associated with
exposure to lead, cadmium, mercury
and arsenic (Järup 2003).

Causes and toxicity of heavy metal
contaminants
HEAVY METAL TOXICITY IS ONE OF THE MAJOR CURRENT ENVIRONMENT HEALTH
PROBLEMS AND IS POTENTIALLY DANGEROUS BECAUSE OF BIO-ACCUMULATION
THROUGH THE FOOD CHAIN (ASCHNER 2002)




               http://www.csuwai.ws/heavymetal/images/
               metal_contamination3.jpg
BIOACCUMULATION AND BIOMAGNIFICATION



        http://www.organicera.com.au/Organics/Bio
        Magnification/tabid/955/Default.aspx
EFFECTS OF LEAD POISONING

       http://trytostayhealthy.blogspot.com/2011/0
       4/lead-poisoning.html
       http://healthandenergy.com/air_pollution_h
       ealth_effects.htm
HAZARDS OF CADMIUM




         http://www.bnl.gov/today/story.asp?ITEM_
         NO=2527
DANGEROUS MERCURY


 http://scienceandtechnology-prem.blogspot.com/2010/07/mercury-   http://pollutionpicture
 poisoning-and-minamata-epidemic.html                             s.blogspot.com/2010
                                                                  /07/minamata-
                                                                  disease-mercury-
                                                                  poisoning-1932.html
ARSENIC
CONVENTIONAL TECHNOLOGIES USED FOR THE
REMOVAL OF HEAVY METAL IONS
                          Disadvantages
1.   Electro-winning      1. Incomplete metal removal
2.   Ion exchange         2. High energy and reagent requirements
3.   Lime precipitation   3. Generation of toxic sludge or waste
                             products
4.   Reverse osmosis
5.   Electro-dialysis
6.   Ultra-filtration
7.   Phytoremediation
THE POTENTIAL OF MICROORGANISMS TO
REMOVE HEAVY METALS IN CONTAMINATED
                SITES

  BIOREMEDIATION
Bioremediation
• Introduction of microbes
into the environment to              Cadmium binding ability of the
restore stability or to
clean up toxic pollutants             blue-green alga Hapalosiphon
• oil spills, heavy metals,           welwitschii Nägel under
pesticides, chemical
wastes, solid waste
disposal (man-made
                                      controlled conditions
plastics and paper
products)

• water and sewage
treatment; reclamation
of polluted water

                              Bi 120 Introduction to Microbiology (mlcdg 2010)
BIOREMEDIATION
• a process that uses naturally         Kinds of bioremediation
  occurring       or      genetically
                                        1. Composting
  engineered microorganisms such
  as yeasts, fungi and bacteria to      2. Bioaugmentation -introduction of a
  transform harmful substances             group of natural microbial strain or a
                                           genetically engineered variant so as to
  into less or nontoxic compounds.         achieve bioremediation.
                                        3. Phytoremediation
• microorganisms break down a
  variety of organic compounds in
  nature to obtain nutrients,
  carbon, and energy for growth
  and survival.
ADVANTAGES AND DISADVANTAGES OF BIOREMEDIATION
  Advantages                         Disadvantages
  1. Eco-friendly, cost-effective,   1. Takes longer compared to
     natural method                     other remedial methods
  2. Al technology targeted to
     remove heavy metals,            2. The techniques are not yet
     radionuclides, xenobiotic          refined for sites with mixtures
     compounds, organic wastes,         of contaminants.
     pesticides, etc. using
     biological means                3. More research is needed to
  3. Used in in-situ conditions         perfect this technology.
BIOREMEDIATION HOLDS
 ENORMOUS PROMISE FOR THE
           FUTURE
(CLEAN UP AND PROTECTION OF
     THE ENVIRONMENT
MICROORGANISMS FOR BIOREMEDIATION




                                                 Fungi
Bacteria                   Algae
                                                 •   Unicellular: yeasts/molds
•   Single celled, with
                           •   Unicellular/mul
    various shapes                               •   Multicellular: mushrooms
                               ticellular
•   Cellular but have no   •   Photosynthetic    •   Saprophytes
    nucleus (prokaryote)   •   Widely
                               distributed       •   Widely distributed
•   Autotroph/chemotroph
•   Stationary/motile
MICROORGANISMS THAT CAN TAKE UP AND
  ACCUMULATE HEAVY METALS
1. bacteria: Sedum alfredii Hance (Xiong et al. 2008), Stenotrophomonas
maltophilia (Parungao et al. 2007), Bacillus circulans strain EB1 (Yilmaz and
   Ensari 2005), and Corynebacterium glutamicum (Choi and Yun 2004)


2. blue-green algae: Nostoc calcicola (Pant 2000), Synechococcus aquatilis
          (Reynaud) strain SY 101 (Vallarta, et al. 1998), and Anacystis nidulans
          (Singh 1985): and
3. microalgae: Tetraselmis suecica (Perez-Rama et al. 2002) and Chlorella
         vulgaris (Carr et al. 1998);
HOW DO THEY DO IT?

1.   The role of cellular structure, storage polysaccharides, cell wall and
     extracellular polysaccharides is evaluated in terms of their potential for
     metal sequestration.
2.    Binding mechanisms, including the key functional groups involved and the
     ion-exchange process. Quantification of metal-biomass interactions is
     fundamental to the evaluation of potential implementation strategies, hence
     sorption isotherms, ion-exchange constants, as well as models used to
     characterize algal biosorption . The sorption behavior (i.e., capacity, affinity)
     of brown algae with various heavy metals is summarized and their relative
     performance is evaluated
BIOSORPTION
• Biosorption technology is based on extensive research work which resulted
  in the discovery of potent metal-binding biomass types.
•   This technology is capable of effectively and economically removing heavy
    metals from industrial aqueous solutions and wastewaters.
•   The metals of sufficiently high values can be recovered and resold.
• These unique biosorbent materials are derived from specific types of
  microbial biomass by a simple process which makes them applicable
    in large-scale sorption processes.
DIRECTIONS OF RESEARCH
•   The bioremediation technology most suitable for a specific site is determined by
    several factors, such as site conditions, indigenous microorganism population,
    and the type, quantity, and toxicity of contaminant chemicals present.
•   Some treatment technologies involve the addition of nutrients to stimulate or
    accelerate the activity of indigenous microbes.
•   Optimizing environmental conditions enhance the growth of microorganisms and
    increase microbial population resulting in improved degradation of hazardous
    substances.
•   However, if the biological activity needed to degrade a particular contaminant is
    not present at the site, suitable microbes from other locations, called exogenous
    microorganisms, can be introduced and nurtured.
•   Other technologies being demonstrated are phytoremediation, or the use of
    plants to clean up contaminated soils and ground water, and fungal remediation,
    which employs white-rot fungus to degrade contaminants.

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Edsc350 review paper for skype 2

  • 1. THE REMOVAL OF HEAVY METALS BY MICROORGANISMS: BIOREMEDIATION AT WORK A Review Paper submitted by MARGARET DE GUZMAN As fulfillment for the requirement in EDSC 350 (Advance Topics in Biology for Teachers)
  • 2.
  • 4. WASTEWATER TREATMENT A polluted creek covered with trash in Manila, Philippines on 01 March 2009. The Department of Environment and Natural Resources reported in 2008 that the Philippines hosts 50 major polluted rivers, with a majority of pollutants coming from domestic waste
  • 6. SOURCES OF HEAVY METALS IN OUR HOMES HEAVY METAL SOURCES & HOW THEY AFFECT US
  • 7. HEAVY METALS • chemical elements with a specific gravity that is at least 5 times the specific gravity of water. The specific gravity of water is 1 at 4°C (39°F). • Some well-known toxic metallic elements with a specific gravity that is 5 or more times that of water are arsenic, 5.7; cadmium, 8.65; iron, 7.9; lead, 11.34; and mercury, 13.546 (Lide 1992).
  • 8. The main threats to human health from heavy metals are associated with exposure to lead, cadmium, mercury and arsenic (Järup 2003). Causes and toxicity of heavy metal contaminants
  • 9. HEAVY METAL TOXICITY IS ONE OF THE MAJOR CURRENT ENVIRONMENT HEALTH PROBLEMS AND IS POTENTIALLY DANGEROUS BECAUSE OF BIO-ACCUMULATION THROUGH THE FOOD CHAIN (ASCHNER 2002) http://www.csuwai.ws/heavymetal/images/ metal_contamination3.jpg
  • 10. BIOACCUMULATION AND BIOMAGNIFICATION http://www.organicera.com.au/Organics/Bio Magnification/tabid/955/Default.aspx
  • 11. EFFECTS OF LEAD POISONING http://trytostayhealthy.blogspot.com/2011/0 4/lead-poisoning.html http://healthandenergy.com/air_pollution_h ealth_effects.htm
  • 12. HAZARDS OF CADMIUM http://www.bnl.gov/today/story.asp?ITEM_ NO=2527
  • 13. DANGEROUS MERCURY http://scienceandtechnology-prem.blogspot.com/2010/07/mercury- http://pollutionpicture poisoning-and-minamata-epidemic.html s.blogspot.com/2010 /07/minamata- disease-mercury- poisoning-1932.html
  • 15. CONVENTIONAL TECHNOLOGIES USED FOR THE REMOVAL OF HEAVY METAL IONS Disadvantages 1. Electro-winning 1. Incomplete metal removal 2. Ion exchange 2. High energy and reagent requirements 3. Lime precipitation 3. Generation of toxic sludge or waste products 4. Reverse osmosis 5. Electro-dialysis 6. Ultra-filtration 7. Phytoremediation
  • 16. THE POTENTIAL OF MICROORGANISMS TO REMOVE HEAVY METALS IN CONTAMINATED SITES BIOREMEDIATION
  • 17. Bioremediation • Introduction of microbes into the environment to Cadmium binding ability of the restore stability or to clean up toxic pollutants blue-green alga Hapalosiphon • oil spills, heavy metals, welwitschii Nägel under pesticides, chemical wastes, solid waste disposal (man-made controlled conditions plastics and paper products) • water and sewage treatment; reclamation of polluted water Bi 120 Introduction to Microbiology (mlcdg 2010)
  • 18. BIOREMEDIATION • a process that uses naturally Kinds of bioremediation occurring or genetically 1. Composting engineered microorganisms such as yeasts, fungi and bacteria to 2. Bioaugmentation -introduction of a transform harmful substances group of natural microbial strain or a genetically engineered variant so as to into less or nontoxic compounds. achieve bioremediation. 3. Phytoremediation • microorganisms break down a variety of organic compounds in nature to obtain nutrients, carbon, and energy for growth and survival.
  • 19. ADVANTAGES AND DISADVANTAGES OF BIOREMEDIATION Advantages Disadvantages 1. Eco-friendly, cost-effective, 1. Takes longer compared to natural method other remedial methods 2. Al technology targeted to remove heavy metals, 2. The techniques are not yet radionuclides, xenobiotic refined for sites with mixtures compounds, organic wastes, of contaminants. pesticides, etc. using biological means 3. More research is needed to 3. Used in in-situ conditions perfect this technology.
  • 20. BIOREMEDIATION HOLDS ENORMOUS PROMISE FOR THE FUTURE (CLEAN UP AND PROTECTION OF THE ENVIRONMENT
  • 21. MICROORGANISMS FOR BIOREMEDIATION Fungi Bacteria Algae • Unicellular: yeasts/molds • Single celled, with • Unicellular/mul various shapes • Multicellular: mushrooms ticellular • Cellular but have no • Photosynthetic • Saprophytes nucleus (prokaryote) • Widely distributed • Widely distributed • Autotroph/chemotroph • Stationary/motile
  • 22. MICROORGANISMS THAT CAN TAKE UP AND ACCUMULATE HEAVY METALS 1. bacteria: Sedum alfredii Hance (Xiong et al. 2008), Stenotrophomonas maltophilia (Parungao et al. 2007), Bacillus circulans strain EB1 (Yilmaz and Ensari 2005), and Corynebacterium glutamicum (Choi and Yun 2004) 2. blue-green algae: Nostoc calcicola (Pant 2000), Synechococcus aquatilis (Reynaud) strain SY 101 (Vallarta, et al. 1998), and Anacystis nidulans (Singh 1985): and 3. microalgae: Tetraselmis suecica (Perez-Rama et al. 2002) and Chlorella vulgaris (Carr et al. 1998);
  • 23. HOW DO THEY DO IT? 1. The role of cellular structure, storage polysaccharides, cell wall and extracellular polysaccharides is evaluated in terms of their potential for metal sequestration. 2. Binding mechanisms, including the key functional groups involved and the ion-exchange process. Quantification of metal-biomass interactions is fundamental to the evaluation of potential implementation strategies, hence sorption isotherms, ion-exchange constants, as well as models used to characterize algal biosorption . The sorption behavior (i.e., capacity, affinity) of brown algae with various heavy metals is summarized and their relative performance is evaluated
  • 24. BIOSORPTION • Biosorption technology is based on extensive research work which resulted in the discovery of potent metal-binding biomass types. • This technology is capable of effectively and economically removing heavy metals from industrial aqueous solutions and wastewaters. • The metals of sufficiently high values can be recovered and resold. • These unique biosorbent materials are derived from specific types of microbial biomass by a simple process which makes them applicable in large-scale sorption processes.
  • 25. DIRECTIONS OF RESEARCH • The bioremediation technology most suitable for a specific site is determined by several factors, such as site conditions, indigenous microorganism population, and the type, quantity, and toxicity of contaminant chemicals present. • Some treatment technologies involve the addition of nutrients to stimulate or accelerate the activity of indigenous microbes. • Optimizing environmental conditions enhance the growth of microorganisms and increase microbial population resulting in improved degradation of hazardous substances. • However, if the biological activity needed to degrade a particular contaminant is not present at the site, suitable microbes from other locations, called exogenous microorganisms, can be introduced and nurtured. • Other technologies being demonstrated are phytoremediation, or the use of plants to clean up contaminated soils and ground water, and fungal remediation, which employs white-rot fungus to degrade contaminants.