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ULTRASONOGRAPHY INTRODUCTION

• Ultrasonograpy is a non-invasive imaging modality using high
  frequency sound waves.
• Used
       -For   diagnostic
                 - Tomographic imaging of soft tissues
                 - to quantitate the functional movement of organ
                 - to measure the size of the organ`
                 - to study the patterns of blood flow
                 - to assess fetal age
       - For therapeutic purpose—
                    - HIFU- Knifeless surgery
                    - Physiotherapy
                    - Lithotripsy
• The human ear can detect
  frequencies in the range of
  20 -20000 Hz.

• Sound above this range is
  known as Ultrasound.

• Used for both diagnostic and
  therapeutic purposes.

• Most diagnostic instruments
  use sound in the range of 1-10
  MHz
• THE IMAGE is displayed
  in various shades of grey
                                      PULSE-ECHO ” PRINCIPLE
  depending on tissue             Piezo- Electric crystal (Lead Zirconate
  density                                     Titanate- PZT)

  e.g. bone appears white
  and fluid appears black.

• The number of shades of
  grey displayed by the
  machine is around 256,
  but the human eye can
  perceive only 16 shades of
  grey. This improves the      ULTRASONOGRAPHY DEMONSTRATES
                               THE STRUCTURE OF TISSUE RATHER
  resolution of the picture.   THAN TISSUE TYPE.
A ) ULTRASOUND WAVES NEED MEDIUM FOR TRAVELLING
• Velocity (V= ν ) of sound is constant in soft body
  tissue but increase with intensity of medium.
B) Attenuation / reduction in intensity
•   US beam is attenuated as it travels through tissues
•    Echoes reflected back towards the transducer are also
    attenuated.
•   Factors contributing to Attenuation are:


1. Absorption:
• Energy in ultrasound beam is absorbed by tissues and is
  converted into heat.
• It is the basis of therapeutic ultrasound.
2. Acoustic impedance- ‘Z’
 It is the resistance offered by tissues to the sound waves.
 Z tissue = Tissue density x Velocity of sound in that Tissue
3. Reflection: It is basis of diagnostic ultrasound
• Sound Waves Are Reflected Back Towards transducers
  giving rise to echo.
• It Occurs
   – if two adjuscent tissues have different Z
   – occurs at patient transducer interface - poor coupling
   – incidence angle




   – “Z” (TISSUE DENSITY) determines the % of the reflected
     beam as it passed from one tissue to another.
Sound reflection at various interfaces
3. Scattering:
• It occurs when the beam encounters irregular interface.
• Angle of US beam interacting with this interface results
  in scattering in all direction.
• CAUSES ARTIFACTS
4. Refraction:
                         • sound waves bend at CURVED
                           interfaces

                         • Angle of incidence.

                         • Affects both transmission &
                           reflection

                         • Can create artifacts

5. Transmission:
 When [Z] of tissues at interface are same- allow
  penetration to depth
A.

COMPONENTS OF AN ULTRASONOGRAPHIC MACHINE
•    TRANSDUCERS
•    CONTROL PANEL
•    COMPUTER
•    PRINTER
•    COUPLING GEL
     TRANSDUCER
•    Function: To send and receive signals
•    Piezo – electric crystal (Lead zerconate titanate)
•    FREQUENCY- Resolution Vs Penetration
•    Lower the frequency, lesser resolution, greater
     penetration.
•    Greater the frequency, greater resolution, lesser
     penetration.
TYPES OF TRANSDUCERS
LINEAR ARRAY TRANSDUCERS Thin
rectangular clips lined side by
side (60-256 crystal in line)

Beam produced is rectangular




             Applications: superficial structures
                      Vascular, Small Parts
 CURVI-LINEAR TYPE
 Shaped in a curve –
 Trapezoidal view –


 both superficial & deeper
  structures are imaged.
SECTOR TYPE
• Single crystal oscillates to
  provide fan shaped beam
• Small size, more
  maneuverability
• Used for thoracic and
  abdominal organs through
  small contact area




Applications
Cardiac , Abdomen , Transcranial , Vascular
MISCELLANEOUS TRANSDUCERS
• US TRANSDUCER with a Biopsy probe
  guide




• Endoscopic - Esophageal probe
Image Display Modes

A-Mode (Amplitude mode)
  Single Ultrasound beam is used
  The returning echoes are shown
  as peaks along the horizontal
  axis.
  The height of the peak is directly
  proportional to the strength of
  the echo.
  Gives information about the
  organ boundaries.
B-Mode (Brightness mode):
• Multiple Ultrasound beams
  are used.
• Returning echo is depicted
  as the dots.
• Brightness of the dot is
  directly proportional to the
  strength of the echo.
• So, is also called as the
  “Grey- Scale imaging”.
• 2D image –
• most popular mode of
  display
Real-Time B- Mode Ultrasonography
Real-time B-mode
scanners display a moving
gray scale image of cross
sectional anatomy
M- Mode (Time-Motion mode)
• Single Ultrasound beam is used.
• Returning echoes depicted as dots.
• Position of dot will denote depth of
  organ -- along the vertical axis.
• Moving structure (Time) on
  horizontal axis.
• Brightness of dot denote the
  strength of echo.
• It is used
   – Echocardiography-
SCANNING PROCEDURE
• First the organ to be scanned
  is decided- case history,
  complaint,          symptoms,
  clinical examination, lab
  examination,       radiological
  examination etc

• With the knowledge of the
  topographic anatomy, use
  the     ‘acoustic   window’
  (Easiest and the nearest site
  for passing the ultrasound
  wave into the desired
  organs)
DORSAL RECUMBANCY - abdomen
Selection of transducer
• It depends on
     – Size of animals
     – Depth of organ.

– Objective : Choose the highest frequency that will
  penetrate to the depth needed for the particular
    examination and gives highest resolution.

•   Small dog, cats  7.5 to 10.0 MHz
•   Medium dogs           5 MHz
•   Large dogs            3.5 to 5.0 MHz
•   Large animals         2.0 to 3.5 MHz
Scanning controls
                        • Near /Far Gain:- alter
Basic set of controls
                          amplification of returning
                          echoes

                        • Time compensation
                          gain:

                        • Depth- determine the depth
                          of US image

                        • Freeze- real time image can be
                          temporarily frozen
IMAGE INTERPRETATION
• Hyper-echoic / Echogenic
  /Echo Rich- WHITE AREAS
   – Given by highly reflective
     interface such as bone or
     air.

• Hypo- Echoic / Echo poor-
  GREY AREAS
   – Given by interface of
    moderate reflection.

– Anechoic/echo lucent/echo
  free- BLACK AREAS
  •    Denotes the complete
    transmission of the sound
    as through the fluids.

– Iso echoic-                     THE STRUCTURE OF TISSUE
                                  RATHER THAN TISSUE TYPE.
•TISSUES IN ORDER OF             Interpretation based on
INCREASING ECHOGENECITY
                                 texture of organ
•Bile, urine
•Renal medulla                     Uniform/ regular
•Muscle                            /homogenous
•Renal cortex
•Liver
                                   Non-uniform/ irregular/
•Storage fat
                                   non-homogenous
•Spleen
•Prostate
•Renal sinus                       Fine Granular / Coarse
•Structural fat, vessel walls      Granular
•Bone, gas, organ
boundaries, calculi
IMAGING OF REPRODUCTIVE TRACT
           objectives
 –   Presence or absence of a pregnancy
 –   Identify the location of the pregnancy –
     intrauterine or extrauterine.
  – Assess the growth and development of the fetus.
  – Placental localization-
  – Assess the amount of liquor amnii.
  – Assess the fetal age.
Time of Sonographic recognition of
      canine fetal structures
3.TESTES
• Topography:
  – Testicles are superficial structures- high freq. transducer used.
  – Non scrotal testicles search should begin in inguinal region.
• NORMAL APPEARANCE
  – well circumscribed, smooth outline, oval in shape
  – parenchyma moderately echogenic

                Sagital image                    Transverse image
IMAGING OF THE THORACIC CAVITY

• Dis advantages: Not easy to image.
   – the rib cage
   – air within the lungs

• Transducer is placed
   – on Inter costal Spaces,
   – behind the xiphisternum
   – at the thoracic inlet.
DUPLEX DOPPLER ULTRASONOGRAPHY
• Involves the simultaneous use of real time
  B-Mode imaging and pulsed doppler ultrasound

PRINCIPLE OF DOPPLER: Blood cells moving towards
   transducer give bright echoes & which move
   away from transducer give weak echoes.
   -Towards transducer – red / yellow / orange
   -Away from transducer – blue/green
FORMS OF DOPPLER
• Pulsed Doppler:
• Continuous Doppler:

Uses:
-to identify structures by the presence or absence of
  blood flow to organ
-to detect thrombus or clot in blood vessel
-to study direction of blood flow & associated
  abnormality
Advantages of ultrasound
• It is a non- invasive diagnostic technique
• Differentiation of soft tissue abnormalities
• Visualization of intra- organ anomaly
• Technique can be performed on any patient
  i.e., old, dyspnoeic and comatose patients
• No known harmful effects
• Visualiztion of Biopsy being taken.
Constraints
• Ultrasound imaging is not suitable
   – Examination of tissues lying below bones
   – Air containing organs – lungs, intestine etc
• Clipping / shaving of hairs –aesthetic value
• Application of coupling medium such as mineral
  oil, aqueous gel - desquamation of cells leading to
  metastasis.
• High capital investment involved
• Technical expertise

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Ultrsonography Principle and application

  • 1. ULTRASONOGRAPHY INTRODUCTION • Ultrasonograpy is a non-invasive imaging modality using high frequency sound waves. • Used -For diagnostic - Tomographic imaging of soft tissues - to quantitate the functional movement of organ - to measure the size of the organ` - to study the patterns of blood flow - to assess fetal age - For therapeutic purpose— - HIFU- Knifeless surgery - Physiotherapy - Lithotripsy
  • 2. • The human ear can detect frequencies in the range of 20 -20000 Hz. • Sound above this range is known as Ultrasound. • Used for both diagnostic and therapeutic purposes. • Most diagnostic instruments use sound in the range of 1-10 MHz
  • 3. • THE IMAGE is displayed in various shades of grey PULSE-ECHO ” PRINCIPLE depending on tissue Piezo- Electric crystal (Lead Zirconate density Titanate- PZT) e.g. bone appears white and fluid appears black. • The number of shades of grey displayed by the machine is around 256, but the human eye can perceive only 16 shades of grey. This improves the ULTRASONOGRAPHY DEMONSTRATES THE STRUCTURE OF TISSUE RATHER resolution of the picture. THAN TISSUE TYPE.
  • 4. A ) ULTRASOUND WAVES NEED MEDIUM FOR TRAVELLING • Velocity (V= ν ) of sound is constant in soft body tissue but increase with intensity of medium.
  • 5. B) Attenuation / reduction in intensity • US beam is attenuated as it travels through tissues • Echoes reflected back towards the transducer are also attenuated. • Factors contributing to Attenuation are: 1. Absorption: • Energy in ultrasound beam is absorbed by tissues and is converted into heat. • It is the basis of therapeutic ultrasound.
  • 6. 2. Acoustic impedance- ‘Z’  It is the resistance offered by tissues to the sound waves.  Z tissue = Tissue density x Velocity of sound in that Tissue
  • 7. 3. Reflection: It is basis of diagnostic ultrasound • Sound Waves Are Reflected Back Towards transducers giving rise to echo. • It Occurs – if two adjuscent tissues have different Z – occurs at patient transducer interface - poor coupling – incidence angle – “Z” (TISSUE DENSITY) determines the % of the reflected beam as it passed from one tissue to another.
  • 8. Sound reflection at various interfaces
  • 9. 3. Scattering: • It occurs when the beam encounters irregular interface. • Angle of US beam interacting with this interface results in scattering in all direction. • CAUSES ARTIFACTS
  • 10. 4. Refraction: • sound waves bend at CURVED interfaces • Angle of incidence. • Affects both transmission & reflection • Can create artifacts 5. Transmission:  When [Z] of tissues at interface are same- allow penetration to depth
  • 11. A. COMPONENTS OF AN ULTRASONOGRAPHIC MACHINE • TRANSDUCERS • CONTROL PANEL • COMPUTER • PRINTER • COUPLING GEL TRANSDUCER • Function: To send and receive signals • Piezo – electric crystal (Lead zerconate titanate) • FREQUENCY- Resolution Vs Penetration • Lower the frequency, lesser resolution, greater penetration. • Greater the frequency, greater resolution, lesser penetration.
  • 12. TYPES OF TRANSDUCERS LINEAR ARRAY TRANSDUCERS Thin rectangular clips lined side by side (60-256 crystal in line) Beam produced is rectangular Applications: superficial structures Vascular, Small Parts
  • 13.  CURVI-LINEAR TYPE  Shaped in a curve –  Trapezoidal view –   both superficial & deeper structures are imaged.
  • 14. SECTOR TYPE • Single crystal oscillates to provide fan shaped beam • Small size, more maneuverability • Used for thoracic and abdominal organs through small contact area Applications Cardiac , Abdomen , Transcranial , Vascular
  • 15. MISCELLANEOUS TRANSDUCERS • US TRANSDUCER with a Biopsy probe guide • Endoscopic - Esophageal probe
  • 16. Image Display Modes A-Mode (Amplitude mode) Single Ultrasound beam is used The returning echoes are shown as peaks along the horizontal axis. The height of the peak is directly proportional to the strength of the echo. Gives information about the organ boundaries.
  • 17. B-Mode (Brightness mode): • Multiple Ultrasound beams are used. • Returning echo is depicted as the dots. • Brightness of the dot is directly proportional to the strength of the echo. • So, is also called as the “Grey- Scale imaging”. • 2D image – • most popular mode of display
  • 18. Real-Time B- Mode Ultrasonography Real-time B-mode scanners display a moving gray scale image of cross sectional anatomy
  • 19. M- Mode (Time-Motion mode) • Single Ultrasound beam is used. • Returning echoes depicted as dots. • Position of dot will denote depth of organ -- along the vertical axis. • Moving structure (Time) on horizontal axis. • Brightness of dot denote the strength of echo. • It is used – Echocardiography-
  • 20. SCANNING PROCEDURE • First the organ to be scanned is decided- case history, complaint, symptoms, clinical examination, lab examination, radiological examination etc • With the knowledge of the topographic anatomy, use the ‘acoustic window’ (Easiest and the nearest site for passing the ultrasound wave into the desired organs)
  • 21.
  • 23. Selection of transducer • It depends on – Size of animals – Depth of organ. – Objective : Choose the highest frequency that will penetrate to the depth needed for the particular examination and gives highest resolution. • Small dog, cats  7.5 to 10.0 MHz • Medium dogs  5 MHz • Large dogs  3.5 to 5.0 MHz • Large animals  2.0 to 3.5 MHz
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  • 26. Scanning controls • Near /Far Gain:- alter Basic set of controls amplification of returning echoes • Time compensation gain: • Depth- determine the depth of US image • Freeze- real time image can be temporarily frozen
  • 27. IMAGE INTERPRETATION • Hyper-echoic / Echogenic /Echo Rich- WHITE AREAS – Given by highly reflective interface such as bone or air. • Hypo- Echoic / Echo poor- GREY AREAS – Given by interface of moderate reflection. – Anechoic/echo lucent/echo free- BLACK AREAS • Denotes the complete transmission of the sound as through the fluids. – Iso echoic- THE STRUCTURE OF TISSUE RATHER THAN TISSUE TYPE.
  • 28. •TISSUES IN ORDER OF  Interpretation based on INCREASING ECHOGENECITY texture of organ •Bile, urine •Renal medulla  Uniform/ regular •Muscle /homogenous •Renal cortex •Liver  Non-uniform/ irregular/ •Storage fat non-homogenous •Spleen •Prostate •Renal sinus  Fine Granular / Coarse •Structural fat, vessel walls Granular •Bone, gas, organ boundaries, calculi
  • 29. IMAGING OF REPRODUCTIVE TRACT objectives – Presence or absence of a pregnancy – Identify the location of the pregnancy – intrauterine or extrauterine. – Assess the growth and development of the fetus. – Placental localization- – Assess the amount of liquor amnii. – Assess the fetal age.
  • 30. Time of Sonographic recognition of canine fetal structures
  • 31. 3.TESTES • Topography: – Testicles are superficial structures- high freq. transducer used. – Non scrotal testicles search should begin in inguinal region. • NORMAL APPEARANCE – well circumscribed, smooth outline, oval in shape – parenchyma moderately echogenic Sagital image Transverse image
  • 32. IMAGING OF THE THORACIC CAVITY • Dis advantages: Not easy to image. – the rib cage – air within the lungs • Transducer is placed – on Inter costal Spaces, – behind the xiphisternum – at the thoracic inlet.
  • 33. DUPLEX DOPPLER ULTRASONOGRAPHY • Involves the simultaneous use of real time B-Mode imaging and pulsed doppler ultrasound PRINCIPLE OF DOPPLER: Blood cells moving towards transducer give bright echoes & which move away from transducer give weak echoes. -Towards transducer – red / yellow / orange -Away from transducer – blue/green
  • 34. FORMS OF DOPPLER • Pulsed Doppler: • Continuous Doppler: Uses: -to identify structures by the presence or absence of blood flow to organ -to detect thrombus or clot in blood vessel -to study direction of blood flow & associated abnormality
  • 35. Advantages of ultrasound • It is a non- invasive diagnostic technique • Differentiation of soft tissue abnormalities • Visualization of intra- organ anomaly • Technique can be performed on any patient i.e., old, dyspnoeic and comatose patients • No known harmful effects • Visualiztion of Biopsy being taken.
  • 36. Constraints • Ultrasound imaging is not suitable – Examination of tissues lying below bones – Air containing organs – lungs, intestine etc • Clipping / shaving of hairs –aesthetic value • Application of coupling medium such as mineral oil, aqueous gel - desquamation of cells leading to metastasis. • High capital investment involved • Technical expertise