induce breeding pemijahan ikan lele smkn 1 suboh situbondo

1. GERMINATING OF THE
CATFISH
(Clarias gariepinus)
USING INDUCE BREEDING
by :
fishery
SMKN 1 SUBOH
SITUBONDO – EAST JAVA

2. Breeding of Clarias gariepinus
Background
Hipofisation
Donor Fish
Recipient Fish
Fasting
Material and THE MATERIALS Instruments of Hipofisation
Taking of Hipofisation
Making of Hipofisation Liquid
Broodstock Injection
Striping of Female Broodstock Ovum
Surgical Operation of Male Broodstock Sperm
Mixing of Ovum and Sperm
Spreading Into Hatchery
Counting of The Hatchery Rate

3. • THE BACKGROUND
 Fish is one of important source
protein for human being
 Inhibiting factor: lack of quality
germ
 Causal factor: fish usually
breeds naturally
 Applying of aquaculture
biotechnology: induce breeding
technique, using the method of
hipofisation technique
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4. HIPOFISATION TECNIQUE
1. is the stimulated spawning using
the extract of hipofisa gland.
2. Purpose :
3. - to stimulate spawning of the fish
,
4. - to intensify the seeds production
5. to crossbreed by using induced
fertilizing
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5. PREPARING A DONOR
FISH
• CRITERIA
♠ From the same species, or
fr om univer sal donor fish
(Example : gold fish)
♠ Sexually Mature
♠ Prefer ably from the male
br ood
♠ Healthy and disease fr ee
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♠ T he weight is known

6. PREPARING THE RECIPIENT
FISH
Sexually Mature
From Aquaculture Fish (Not wild fish)
Healthy and normal (having no
physical defect)
The age meets the
requirement/category
Not A discarded Fish
Having superior
features/characteristic
The weight is known
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7. DIFFERENTIATING BETWEEN SEX
OF CATFISH BROODSTOCK
Male : in its sexual organ there is a
protrusion pointed as the penis of male
catfish
Female : near its anus, there is a small
protrusion marked by a reddish hole as
its sexual organ of female catfish
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8. female
male
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9. FEMALE BROODSTOCK MATURITY
IS SIGNED BY
The share of sex organ is
spherical reddish, the hole is
getting bigger
Its movement is slower than
before
The colour of skin is brighter than
before
The stomach feels soft when
touched
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10. MALE BROODSTOCK MATURE
IS SIGNED BY
ITS SEXUAL ORGAN LOOKS
POINTED AND REDDISH
THE STOMACH IS SLENDER
THE SKIN COLOUR IS DARKER
THAN BEFORE
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11. FASTING STAGE
MEANING : FASTING THE FISH FOR A
SEVERAL HOURS BEFORE BREEDING
PURPOSE :
•
- REDUCING FAT AND THE FISH
FECES THAT CAN DECREASE THE
WATER QUALITY
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12. THE MATERIALS
•
DONOR FISH
AQUABIDEST
ALCOHOL
CLEAR WATER
COTTON / TISSUE
FILTER PAPER
SOLUTION OF 0,9% NaCl
(Physiological Salt)
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13. Tools
TISSUE GRINDER
CENTRIFUGE
PINSET COTTON
MANCHETE
SPUIT AND SYRINGE
FISH CUSHION
FILTER PAPER
SCALE
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14. MEASURING THE DOSAGE
Recipient female 1 kg, dosage 1,5
____meaning it needs the donor fish as
weight as 1,5 kg
Recipient male 0, 5 kg , dosage 1____
meaning it needs the donor fish as weight
as 0,5 kg
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15. TAKING THE HIPOFISA
GLAND
The head is cut
Split from under the nose till
separate
Taking the brain carefully using a
pinset
Taking the hipofisa gland
carefully using the pinset
Cleaning with filter paper
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16. Back Next
The head is cut

17. gambar
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Split from under the nose till separate

18. gambar
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Taking the brain

19. gambar
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Taking the hipofisa gland

20. EXTRACTING THE HIPOFISA
GLAND
Hipofisa gland is attached to the
tissue grinder and blended until
tender or done
Add 2 cc of aquabidest
Sett the hipofisa using centrifuge for 2
minutes
Let the hipofisa gland be in the
reaction tube for ±1minute
Taking the clear upper layer using a
spuit
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21. gambar
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22. gambar
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Add 2 cc of aquabidest

23. gambar
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Dissolve the hypofisa gland using the spuit

24. gambar
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ut it in the reaction tube and centrifuge

25. gambar
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Let the hypofisa gland

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Take the clear layer

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The way of injecting

28. DETERMINING THE DOSAGE OF
OVAPRIM HORMON
• The ovaprim hormon dosage is :
• 0,5 ml/kg brood and diluted with 1
ml of aquadest

29. The male catfish brood is put then operate it

30. Lift the sperm bag

31. Slice it into pieces

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Prepare the female catfish egg cell

33. Mixing the egg and sperm of
broodstock
Pour the sperm little by little into a
bowl which is filled with the eggs,
then stir slowly using chicken feather
that has been sterilized
Stir until all of the eggs mixed
perfectly
Wash off the eggs using aquadest
Then spread it into hatchery
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34. Back Next
Mix the egg cell with the sperm

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Wash it using mineral water

36. Spreading the fish egg
Preparing a hatchery tank
Fill with fresh water and the height is 30 cm
Aeration for several hours before, to increase the
dissolved oxygen
Drop adequate methylene blue to avoid the fungus
attack
If the temperature in the media is enough, the
eggs are ready to spread
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37. Back Next
Spred into the hatching container

38. Counting of fecundity catfish
eggs
• Gravimetric methode
• This method is also called as the weight
method.
• Fecundity
= (The egg total weight : The
egg sample weight) x The amount of eggs (in
sample)
• F = Fecundity
• W = The egg total weight
• w = The egg sample weight
• N = The amount of eggs (in sample)
•

39. Management of water quality in the
hatchery of catfish egg
Because the egg that does not hatch
decays faster , it causes the water
quality decreases, so the water in
hatchery must be circulated
continuously
After most of the eggs have hatched,
the hatchery is cleaned from the eggs
that do not hatch
Technical requirement: ph 7-8, water
temperature 28-29oC
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40. Keeping the larva of Clarias sp
The egg will hatch in 24 hours
For one day old, larva of clarias sp.
needn’t feeding because it still has its
own food
For 4 day old, give it an egg yolk or
Tubifex
For 7 day old give it natural food as
artemia and tubifex
Maintain the water quality by
continuous circulation
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41. LARVA FEEDING
the natural food usually given is
artemia or tubifex
The artificial food : powder food
200# or from the egg yolk
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42. Counting the hatching rate
• Sampling method
• Take the homogen larva in sampling way using a
measurement glass
• Total of larva= Total of sampling egg x the
volume of water in the measurement glass
• The rate of hatching = total of larva x 100%
•
fecundity
•
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