7. Blood-based biomarkers
Potential sources
Platelets
Small, anuclear fragments
derived from megakaryocytes in
the bone marrow
Platelets
Respond to
neurotransmitters
Enzymes responsible
for generating the Aβ
peptide.
High concentration of
amyloid precursor
protein (APP)
8. Methodology for identifying Biomarkers
Validation of Cooccurrence of other
pooled analyte
changes consistent
with previous
biomarker studies
Platelet isolation
and membrane
protein enrichment
Quantification of
membrane-enriched
proteome
12. Potential Drug Targets
Metabotropic
glutamate
receptor subtype
5
• Increasing the activation of
mGlu5
Nicotinic
acetylcholine
receptor
• Activation of the α7nAChR
subtype with smallmolecule agonists
M1 Muscarinic
acetylcholine
receptors
• potential to affect multiple
disease pathologies
including amyloid-beta and
phosphorylated tau
13. Potential Drug Targets
• Indirect cholinergic
5Hydroxytryptamine stimulation through
5-hydroxytryptamine
receptors
(5-HT) receptors
6
Norepinephrine
• Noradrenergic
neurons: vulnerable
during aging
Cyclic adenosine
monophosphate
• cAMP signaling is
increased with aging