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Biotechnology Application of scientific and engineering principles for the processing of materials in industrial process to provide goods and services.
Genetic Engineering - makes it possible, through an integrated application of knowledge and techniques of biochemistry, microbiology, genetics and chemical engineering, to draw benefit at the technological level from the properties and capacities of microorganisms and cell culture . Biotechnology
Biotechnology Application potential   -  agriculture, antibiotics, vitamins, vaccines, dairy industries, fermented products like alcoholic & nonalcoholic beverages, production of biogas, sewage treatment plants, bio-fertilizers, tissue culture, genetic engineering, etc.
Gene concept ,[object Object],[object Object],[object Object],[object Object],[object Object]
Gene ,[object Object],[object Object],[object Object],[object Object],promoter Structural gene flank flank upstream downstream 5’ 3’
Nucleic Acids ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Agriculture -plants transformed-insect,disease, and herbicide resistant. -animals treated engineered hormones-produce more milk, leaner meat.
Agriculture -food processors affected by genetic engineering. -shelf-life, storage, food-handling;extended and simplified. -help resist spoilage.
Deoxyribose Nucleic Acid  (DNA) ,[object Object],[object Object],[object Object]
Chemical   components 1. A pentose sugar. 2.  Phosphate group 3. Nitrogenous bases
Nitrogenous bases Purines Pyrimidines Adenine Guanine Thymine Cytosine
Nucleoside and  Nucleotide
Phosphodiester   linkage
Watson   & Crick Model ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
1. Double  Helix
2. Antiparallel strands 3. Major & Minor grooves
[object Object],[object Object],[object Object],[object Object],[object Object],Character of each strand
[object Object],[object Object],Base pairing
Base pairing
Base pairing Complementary nature of strands OH P
 
[object Object],Dimensions
RNA
r ibo n ucleic  a cid 4 bases A  = U  = C  = G  = 3 major types of RNA messenger RNA (mRNA); template for protein synthesis  transfer RNA (tRNA); adaptor molecules that decode the genetic code ribosomal RNA (rRNA); catalyzing the synthesis of proteins C 5 H 10 O 5 A denine U racil C ytosine G uanine Pyrimidine (C 4 N 2 H 4 ) Purine (C 5 N 4 H 4 ) Nucleoside Nucleotide base +  sugar (ribose) base +  sugar +  phosphate RNA RNA structure Thymine (DNA) Uracil (RNA)
Base interactions in RNA Base pairing: U/A/(T) (2 hydrogen bonds) G/C  (3 hydrogen bonds) RNA base composition: A + G = U + C /  Chargaff’s rule does not apply (RNA usually prevails as single strand)  RNA structure: - usually single stranded - many self-complementary regions    RNA commonly exhibits an intricate secondary structure   (relatively short, double helical segments alternated with single stranded regions)  - complex tertiary interactions fold the RNA in its final three dimensional form - the folded RNA molecule is stabilized by interactions (e.g. hydrogen bonds and base stacking)
mRNA ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],Ribosomal RNA
 
tRNA / sRNA
tRNA / sRNA
[object Object],[object Object],[object Object],Genetic Engineering of Animals
[object Object],[object Object],[object Object],[object Object],[object Object],Genetic Engineering of Animals
THE END By:  Kay & Jessica

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1 xii biotechnology

  • 1. Biotechnology Application of scientific and engineering principles for the processing of materials in industrial process to provide goods and services.
  • 2. Genetic Engineering - makes it possible, through an integrated application of knowledge and techniques of biochemistry, microbiology, genetics and chemical engineering, to draw benefit at the technological level from the properties and capacities of microorganisms and cell culture . Biotechnology
  • 3. Biotechnology Application potential - agriculture, antibiotics, vitamins, vaccines, dairy industries, fermented products like alcoholic & nonalcoholic beverages, production of biogas, sewage treatment plants, bio-fertilizers, tissue culture, genetic engineering, etc.
  • 4.
  • 5.
  • 6.
  • 7. Agriculture -plants transformed-insect,disease, and herbicide resistant. -animals treated engineered hormones-produce more milk, leaner meat.
  • 8. Agriculture -food processors affected by genetic engineering. -shelf-life, storage, food-handling;extended and simplified. -help resist spoilage.
  • 9.
  • 10. Chemical components 1. A pentose sugar. 2. Phosphate group 3. Nitrogenous bases
  • 11. Nitrogenous bases Purines Pyrimidines Adenine Guanine Thymine Cytosine
  • 12. Nucleoside and Nucleotide
  • 13. Phosphodiester linkage
  • 14.
  • 15. 1. Double Helix
  • 16. 2. Antiparallel strands 3. Major & Minor grooves
  • 17.
  • 18.
  • 20. Base pairing Complementary nature of strands OH P
  • 21.  
  • 22.
  • 23. RNA
  • 24. r ibo n ucleic a cid 4 bases A = U = C = G = 3 major types of RNA messenger RNA (mRNA); template for protein synthesis transfer RNA (tRNA); adaptor molecules that decode the genetic code ribosomal RNA (rRNA); catalyzing the synthesis of proteins C 5 H 10 O 5 A denine U racil C ytosine G uanine Pyrimidine (C 4 N 2 H 4 ) Purine (C 5 N 4 H 4 ) Nucleoside Nucleotide base + sugar (ribose) base + sugar + phosphate RNA RNA structure Thymine (DNA) Uracil (RNA)
  • 25. Base interactions in RNA Base pairing: U/A/(T) (2 hydrogen bonds) G/C (3 hydrogen bonds) RNA base composition: A + G = U + C / Chargaff’s rule does not apply (RNA usually prevails as single strand) RNA structure: - usually single stranded - many self-complementary regions  RNA commonly exhibits an intricate secondary structure (relatively short, double helical segments alternated with single stranded regions) - complex tertiary interactions fold the RNA in its final three dimensional form - the folded RNA molecule is stabilized by interactions (e.g. hydrogen bonds and base stacking)
  • 26.
  • 27.
  • 28.  
  • 31.
  • 32.
  • 33. THE END By: Kay & Jessica

Notes de l'éditeur

  1. DNA/RNA overview
  2. DNA/RNA overview