This document discusses various concepts in cell biology and biochemistry. It provides multiple choice questions about cell lysis, cell culture conditions, phosphate buffered saline buffering capabilities, and requirements for spectrophotometry assays of protein concentration. The questions cover topics like the goals of cell lysis, conditions needed for cell culture, how RCF and RPM are related in centrifuges, and what proteins must be isolated with for spectrophotometry assays.
Cell lysis could accomplish the following goals EXCEPT Denat.pdf
1. Cell lysis could accomplish the following goals EXCEPT Denaturation of proteins Breaking up
whole cells or tissues Separating cell components Isolation of DNA Question 2 The following
conditions are required for cell culture EXCEPT A culture medium Growth factors Tritonx100
GasesWith the same centrifuge, the relationship between RCF and RPM can be described as
Directly related. Inversely related Notrelated Cannot be determined Question 6 Phosphate
buffered saline can buffer all of the following EXCEPT pH2 salt concentration. osmolarity, protcin
concentrationThe molecular weight of Tris. HCl is 157.6g/mol. If 15.76g of it is added to a final
solution volume of 400ml, the molarity of Tris-HCl in the solution is 0.1M. 1025M 0.25cmMi 0.4M
For a spectrophotometry assay of protein concentrations to work, The protein concentrations must
be high. The proteins must be linked to a dves The proteins must be isolated with a dye. The cells
from which proteins are isolated must be linked to a dye.
