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NexGen Biosolutions Presentation
- 10. PiNK TG MOPS MES* Apparent molecular
Weights for
plus 15% 12% 4-12% 12% 4-12% SDS-PAGE analysis
Band 1 ~175 ~165 ~165 ~165 ~165
Band 2 ~130 ~120 ~120 ~125 ~125
Band 3 ~90 ~80 ~80 ~82 ~82
Band 4 ~70 ~61 ~61 ~62 ~62
Band 5 ~60 ~52 ~52 ~55 ~55
Band 6 ~50 ~42 ~42 ~45 ~45
Band 7 ~40 ~32 ~32 ~34 ~34
Band 8 ~30 ~24 ~24 ~26 ~26
Band 9 ~20 ~17 ~17 ~16 ~16
Band 10 ~15 ~13 ~13 ~12 ~12
Band 11 ~10 ~10 ~10 ~7 ~7
* MES buffer compatible
- 11. Inv. 5800
NEB 7708
Inv. M12
Ferm. 0661
NEB 7703
PiNK Plus
Ferm. 0671
prestained
Tris-Glycine
unstained
15%
200 Average line
250 260 200 175
150 170
175 150 160 130
116 120 130 130
100 110
97.4 90 100
83 85 90
80 80 70
70 70 70
66.3
62 60 60 60 60 60
55.4
55
50 50 50 50
47 50
40
40 40 40
40 40
36.5
35
32
30 30 30 30 30
31
25
25
25
25
20
20
21.5 20 20
20
16.5
15
15
15
15 15 14.4 15
10 10 10 10
10
6.5 6
10
- 12. MOPS
Ferm. 0661
NEB 7708
Ferm. 0671
Inv. 5800
PiNK Plus
NEB 7703
Inv. M12
PiNK plus
PiNK Plus
4-12%
PM 22
prestained
unstained
Average line
GeneDireX
200 260
250
200
200
150 150 ~165 160
150
120 ~120 116
110 120
100 100 97.4
100
85
80
80 ~80
80
66.3
70
70
60 60 ~61 60
55.4 60
~52
50 50
50
50
~42
40
40 40
36.5 40
~32
30 30
31 30
30
25
25 ~24
20
20 21.5
20
20 ~17
14.4 15
15 15
~13 15
10 10
10 ~ 10
10
6
- 13. Ferm. 0661
NEB 7708
Ferm. 0671
PiNK plus
MOPS
Inv. 5800
NEB 7703
Inv. M12
PiNK plus
PiNK plus
12%
PM 22
prestained
unstained
GeneDireX
260
250 200 Average line
200
200
150 ~165
150 160
120 116 150
~120
100 100 110 120
97.4 100
85 ~80
80 80 80
70 66.3
60 70
60 ~61
60 55.4 60
50 ~52
50 50
50
~42
40 40
40
40
36.5
30 30 ~32
31 30
30
25
25
~24
20
20
21.5
20
20
~17
15 15
14.4 15
15
~13
10 10
10 ~ 10 10
6
- 14. Ferm. 0661
Ferm. 0671
PiNK plus
Inv. 5800
NEB 7703
Inv. M12
PiNK plus
PiNK plus
MES
PM 22
PM 22
4-12% prestained
unstained
Fermentas Average line
250 200 260
200
200
150 ~165
160 150
120 150
~125 116 120
100 100
110 100
85 ~82 97.4
80
70 80 80
60 ~62
60 66.3
50 60 60
~55
55.4
50 ~45 50
50
40
40 40
~34 36.5
40
30
31
30
30
25 25 ~26
21.5 20
20 20
~16 20
15 15
15 ~12 14.4
10
10
10 6
~ 7
- 15. MES
Ferm. 0661
Ferm. 0671
PiNK plus
Inv. 5800
12%
NEB 7703
Inv. M12
PiNK plus
PiNK plus
PM 22
PM 22
prestained
unstained
Fermentas 260
250 Average line
200
200 200
150 ~165
150 160
120 150
~125 116
100 100 110 120
97.4
85 ~82 80 100
80
70 66.3 80
~62
60 60
60 ~55 60
55.4
50 50 50
~45 50
36.5
40 40
40 40
~34
30
30 31 30
30
25 25 ~26
21.5
20
20
20 20
~16
14.4 15
15 15
15
10
~12
10
10
6
~ 7