Presentation from the conference ENHANCED GENEPOOL UTILIZATION ‒ Capturing wild relative and landrace diversity for crop improvement, June 2014, Cambridge, UK

1. Tools
Facilitated spatial and ecogeographical germplasm analysis
for efficient PGR conservation and utilization
Mauricio Parra Quijano (ITPGRFA - Programme Coordinator)
Francisco López (ITPGRFA)
Elena Torres (UPM-Spain)
José Iriondo (URJC-Spain)

2. 


3. Funded by:
And with technical support of:

4. Development
Transfer
Training
Building Capacity
National Programs and institutions
from Latin America

5. Idea + methodology development
Theoretical and practical scientific validation
Publication (peer review)
Data preparation R script development
Java / tomcat interface
Tool + documentation
Assistance
CAPFITOGEN tool
release / distribution
GIS and
ecogeographic
approaches on PGR

8. 40° 20’ 33.4’’ N
03° 11’ 52.1’’ W
Or paper maps…
Gazeteers…
Georeferencing software
Old records…
Evaluate the quality of your collecting sites coordinates and locality
description

9. Quality assessment on the georeferencing of the
collecting sites included in passport data

11. Based on: Parra-Quijano et
al., 2012. Gen Res Crop Evol
59(2):205-217

12. Obtaining Ecogeographic Land Characterization (ELC)
maps for a single species or group of related (specific
maps) or unrelated species (generalist maps)

13. Ecogeographic germplasm (collecting sites) characterizations
Up to 105 ecogeographic
variables
Ecogeographic
Characterization
matrix
Georeferenced
Passport data
GIS
Elevation
Annual mean temp
Organic carbon on soil
pH soil
….
….
Y
X

14. Perform ecogeographic characterizations of the
georeferenced collecting sites

15. • Is your collection representative of the species’ environmental adaptive range?
Check the ecogeographical representativeness of your ex situ
collection
GAPS!
REPRESENTED

16. Evaluation of the ecogeographical
representativeness of the germplasm collections

17. • Ocurrence of the highest levels of diversity in terms of average genotypic,
phenotypic or ecogeographic distances among local
Identify where is located the diversity hotspots using phenotypic,
genotypic or ecogeographic data
Maize study
Andean region
Ecuador
Inspired on
Van Zonneveld et al.,
2012. PLoS ONE 7(1):
e29845

18. Obtaining phenotipic, genotypic or ecogeographic
“diversity” maps

19. Select a subset of accessions which represents the species’
ecogeographical range in your collection
A B
A
A
A
A
B
BB
B
C BA
A
B C
A
A
A
AB
B
B
B
CBA
C
A
A
A
A
A
A
A
A
CAB
A
B
CEcogeographic
Core
Collection
Based on Parra Quijano et al., 2011. Crop Science 51:656-666

20. Generation of ecogeographic core collections
based on ELC maps

21. Creating subsets of accessions with high probability to contain
interesting traits
Annual
precipitation
Clay content
Slope
40
4
10% selection
intensity
FIGS subset (by filtering method)
Based on FIGS approach by Mackay,M., Street, K., Bari, A., Endresen, D. and other researchers
Waterlogging tolerance

22. Generation of subsets of breeding interest traits based on
ecogeographic conditions of the collecting sites (Focused
Identification Germplasm Strategy – filtering method)

23. CAPFITOGEN tools offer:
• Avoiding collecting in sites where other have been collecting in past expeditions
• Collecting germplasm in under or non-represented environments
• Collecting germplasm in sites with high probably of traits of interest occurrence

24. GEOQUAL +
ELCmapas +
Representa +
FIGS_R

26. Spanish
Portuguese
English

27.  105 ecogeographical variables to select
 With data available for more than 160 countries
(national work frames)
 Two sub-continental work frames (South and Meso-
America)
 One continent (Europe) and a world-wide work
frame
 1x1, 5x5, 10x10 and 20x20 km resolutions
 Free access, free distribution

28. Programme to Strengthen
National Plant Genetic
Resource Capacities in Latin
America
Phase 1:
2012-2014
Seven tools focused on ex situ conservation
User guides
Three languages (Spanish, English, Portuguese)
Two regional (Latin American) workshops
Four national workshops (Cuba, Spain, Honduras, Uruguay)
About 150 trained technicians

29. Programme to Strengthen
National Plant Genetic
Resource Capacities in Latin
America
Phase 2:
2014-2016
Three new tools focused on in situ conservation
User’s networks
E-Learning methods
Tools on server (internet connection required, no installation)
One final regional (Latin America + Spain + Portugal) workshop
Four national workshops

30. Madrid, Spain, Nov 2013
Bogotá, Colombia, March 2013
Colonia, Uruguay, April 2014
Comayagua, Honduras, Nov 2013
Sancti Spitirus, Cuba, Sept 2013
Florianópolis, Brazil, May 2014
Birmingham, UK, Feb 2014

31. http://www.agrobiodiversidad.org/blog/?p=1039
http://www.planttreaty.org/capfitogen
capfitogen@fao.org
Mauricio.parra@fao.org
Email: