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Cloning within
   Animals
     Nikki Sumner
      Biology 101
       Fall 2011
What exactly is Animal
           Cloning?
• When the term animal cloning
  is used, it is typically referring
  to the artificial creation of an
  identical genetic animal; this
  means that both animals will
  have the exact same DNA
• Cloning does not refer to
  multiple births or normal
  reproduction, including that
  of tissues or organs
Therapeutic Cloning
             vs.
     Reproductive Cloning
• Therapeutic Cloning refers to cloning cells
  from an adult animal or human to use for
  medical reasons, such as research
• Reproductive Cloning refers to making cloned
  animals
How does cloning work?
•Two females are needed to
begin the cloning process
•A body cell is extracted from
the first female, this is where
the DNA is extracted from
•An egg cell is then extracted
from the second female, the
nucleus is extracted from the
egg cell
•Once the nucleus is
extracted from the 2nd
female’s egg cell, the 1st
female’s DNA is placed in the
2nd females empty egg cell
• Once they are
  combined, the egg cell is
  placed into the 1st
  female’s uterus
• The cloned embryo
  will now begin to
  develop
• This is the stage where
  the egg cell could fail
  and not develop into
  the embryo as planned
What are the concerns with
      animal cloning?
• High failure rate
• DNA Mutations
• Developmental
      Problems
  • Ethical Issues
How can cloning be used?
• Repopulate endangered species
• Help aid in animal breeding for those species
  that have difficulty
• Bring back extinct species
• Possible treatment of disorders
What animals have been
                 successfully cloned?
•   Carp was the first fish ever cloned in China in 1963
•   A mouse, named Masha, was the first cloned mammal in 1986, was cloned in
    Russia
•   A horse, named Prometea, was cloned in Italy, in 2003
•   5 twin piglets were cloned in London in 2000
•   A cat, named CC, was the first cloned cat and pet in Texas in 2001
•   Snuppy the dog was first cloned in Korea in 2005
•   Little Nicky, a cat, was the first cloned animal for use in a commercial in California
    in 2004
•   Ombretta, the Mouflan, was the first endangered species to be cloned in Italy, in
    2000
•   Andi, the monkey, was cloned in Oregon in 2000
•   Idaho Gem the Mule was the first animal hybrid to be cloned in 2003
•   Pyrenean Ibex is the first extinct animal to be cloned in 2009
•   Dolly, the sheep, was the first mammal to be cloned from an adult donor in
    Scotland in 1996
Dolly the Sheep
      and
 Jeremy Rifkin
Works Cited
•    "16 Most Notable Cloned Animal | Scienceray." Scienceray | All That Is Science, Astronomy,
           Biology, Chemistry, Mathematics, Physics. Web. 08 Nov. 2011.
           <http://scienceray.com/technology/applied-science/16-most-notable-cloned-
           animal/>.

•    Biotechnology: Friend or Foe?. Films Media Group, 2000. Films On Demand. Web. 7 November
            2011. <http://digital.films.com/PortalPlaylists.aspx?aid=11561&xtid=11530>.

•    Bren, Linda. "Cloning: Revolution Or Evolution in Animal Production?" FDA consumer 2003: 28-
             33. ProQuest Research Library. Web. 7 Nov. 2011
             <http://ezproxy.hacc.edu/login?url=http://search.proquest.com/docview/227040801?
             accountid=11302>.

•    "Cloning Fact Sheet." Oak Ridge National Laboratory. Web. 08 Nov. 2011.
            <http://www.ornl.gov/sci/techresources/Human_Genome/elsi/cloning.shtml#anim
            als>.

•    Erijman, Ariel, Dantes, Ada, Bernheim, Reut, Shifman, Julia M., Peleg, Yoav, Transfer-PCR (TPCR):
            A highway for DNA cloning and protein engineering, Journal of Structural Biology, Volume
            175, Issue 2, August 2011, Pages 171-177, ISSN 1047-8477, 10.1016/j.jsb.2011.04.005.
            (http://www.sciencedirect.com/science/article/pii/S1047847711001109
Works Cited Continued
•   Jurveston, Steve. Cute Clones. 2009. Photograph. Flickr. Flickr. Yahoo, 3 Mar. 2009. Web. 31 Oct.
           2011. <http://www.flickr.com/photos/jurvetson/3327872958/>.

•   Romanov, Michael. "Gene Cloning and DNA Analysis: An Introduction, 5th Ed." The Journal
         of heredity 98.1 (2007): 97-8. ProQuest Research Library. Web. 7 Nov. 2011.

•   Skuds. Scars. 2006. Photograph. Flickr. Flickr. Yahoo, 27 June 2006. Web. 05 Nov. 2011.
           <http://www.flickr.com/photos/skuds/176452661/>.

•   Wheeler, Richard. Benzopyrene DNA Adduct 1JDG.png. 2007. Photograph. Wikipedia. Wikipedia.
          2007. Web. 11 Nov.2011.
          <http://en.wikipedia.org/wiki/File:Benzopyrene_DNA_adduct_1JDG.png>.

•   Wirth, Carl. Gencode. 2010. Photograph. Flickr. Flickr. Yahoo, 29 July 2010. Web. 11 Nov. 2011.
           <http://www.flickr.com/photos/naturalismus/4839348174/>.

•   Ynse. DNA Rendering. 2007. Photograph. Flickr. Flickr. Yahoo, 12 June 2007. Web. 01 Nov. 2011.
           <http://www.flickr.com/photos/ynse/542370154/>.

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Cloning within animals

  • 1. Cloning within Animals Nikki Sumner Biology 101 Fall 2011
  • 2. What exactly is Animal Cloning? • When the term animal cloning is used, it is typically referring to the artificial creation of an identical genetic animal; this means that both animals will have the exact same DNA • Cloning does not refer to multiple births or normal reproduction, including that of tissues or organs
  • 3. Therapeutic Cloning vs. Reproductive Cloning • Therapeutic Cloning refers to cloning cells from an adult animal or human to use for medical reasons, such as research • Reproductive Cloning refers to making cloned animals
  • 4. How does cloning work? •Two females are needed to begin the cloning process •A body cell is extracted from the first female, this is where the DNA is extracted from •An egg cell is then extracted from the second female, the nucleus is extracted from the egg cell •Once the nucleus is extracted from the 2nd female’s egg cell, the 1st female’s DNA is placed in the 2nd females empty egg cell
  • 5. • Once they are combined, the egg cell is placed into the 1st female’s uterus • The cloned embryo will now begin to develop • This is the stage where the egg cell could fail and not develop into the embryo as planned
  • 6. What are the concerns with animal cloning? • High failure rate • DNA Mutations • Developmental Problems • Ethical Issues
  • 7. How can cloning be used? • Repopulate endangered species • Help aid in animal breeding for those species that have difficulty • Bring back extinct species • Possible treatment of disorders
  • 8. What animals have been successfully cloned? • Carp was the first fish ever cloned in China in 1963 • A mouse, named Masha, was the first cloned mammal in 1986, was cloned in Russia • A horse, named Prometea, was cloned in Italy, in 2003 • 5 twin piglets were cloned in London in 2000 • A cat, named CC, was the first cloned cat and pet in Texas in 2001 • Snuppy the dog was first cloned in Korea in 2005 • Little Nicky, a cat, was the first cloned animal for use in a commercial in California in 2004 • Ombretta, the Mouflan, was the first endangered species to be cloned in Italy, in 2000 • Andi, the monkey, was cloned in Oregon in 2000 • Idaho Gem the Mule was the first animal hybrid to be cloned in 2003 • Pyrenean Ibex is the first extinct animal to be cloned in 2009 • Dolly, the sheep, was the first mammal to be cloned from an adult donor in Scotland in 1996
  • 9. Dolly the Sheep and Jeremy Rifkin
  • 10. Works Cited • "16 Most Notable Cloned Animal | Scienceray." Scienceray | All That Is Science, Astronomy, Biology, Chemistry, Mathematics, Physics. Web. 08 Nov. 2011. <http://scienceray.com/technology/applied-science/16-most-notable-cloned- animal/>. • Biotechnology: Friend or Foe?. Films Media Group, 2000. Films On Demand. Web. 7 November 2011. <http://digital.films.com/PortalPlaylists.aspx?aid=11561&xtid=11530>. • Bren, Linda. "Cloning: Revolution Or Evolution in Animal Production?" FDA consumer 2003: 28- 33. ProQuest Research Library. Web. 7 Nov. 2011 <http://ezproxy.hacc.edu/login?url=http://search.proquest.com/docview/227040801? accountid=11302>. • "Cloning Fact Sheet." Oak Ridge National Laboratory. Web. 08 Nov. 2011. <http://www.ornl.gov/sci/techresources/Human_Genome/elsi/cloning.shtml#anim als>. • Erijman, Ariel, Dantes, Ada, Bernheim, Reut, Shifman, Julia M., Peleg, Yoav, Transfer-PCR (TPCR): A highway for DNA cloning and protein engineering, Journal of Structural Biology, Volume 175, Issue 2, August 2011, Pages 171-177, ISSN 1047-8477, 10.1016/j.jsb.2011.04.005. (http://www.sciencedirect.com/science/article/pii/S1047847711001109
  • 11. Works Cited Continued • Jurveston, Steve. Cute Clones. 2009. Photograph. Flickr. Flickr. Yahoo, 3 Mar. 2009. Web. 31 Oct. 2011. <http://www.flickr.com/photos/jurvetson/3327872958/>. • Romanov, Michael. "Gene Cloning and DNA Analysis: An Introduction, 5th Ed." The Journal of heredity 98.1 (2007): 97-8. ProQuest Research Library. Web. 7 Nov. 2011. • Skuds. Scars. 2006. Photograph. Flickr. Flickr. Yahoo, 27 June 2006. Web. 05 Nov. 2011. <http://www.flickr.com/photos/skuds/176452661/>. • Wheeler, Richard. Benzopyrene DNA Adduct 1JDG.png. 2007. Photograph. Wikipedia. Wikipedia. 2007. Web. 11 Nov.2011. <http://en.wikipedia.org/wiki/File:Benzopyrene_DNA_adduct_1JDG.png>. • Wirth, Carl. Gencode. 2010. Photograph. Flickr. Flickr. Yahoo, 29 July 2010. Web. 11 Nov. 2011. <http://www.flickr.com/photos/naturalismus/4839348174/>. • Ynse. DNA Rendering. 2007. Photograph. Flickr. Flickr. Yahoo, 12 June 2007. Web. 01 Nov. 2011. <http://www.flickr.com/photos/ynse/542370154/>.