1. ACIB – UAB Workshop
Systems Biology for Metabolic and Cell Engineering
Barcelona 27-28 May 2014
Begin: May 27, 1:00 pm
End: May 28, 5:00 pm
Venue
Casa Convalescència
Sant Antoni Ma Claret, 171
08041-Barcelona
http://www.uab-casaconvalescencia.org/en/index.php?lg=en

2. Programme
May, 27th
13:00-14:00 Registration
14:00-14:10 Welcome & Introduction P. Ferrer & D. Mattanovich
(UAB & ACIB/BOKU)
14:10-14:55 Plenary lecture: Molecular engineering of
transglucosidases for synthetic applications
Pierre Monsan (INSA
Toulouse)
Session 1 Mammalian cell systems
14:55-15:20 Production of VLPs by transient transfection of HEK293
cells
F. Gòdia (UAB)
15:20-15:45 The era of genome science for CHO: from blackbox
to design and art
N. Borth (ACIB/BOKU)
15:45-16:05 Optimizing transient gene expression of therapeutic,
recombinant proteins in mammalian cells
J.L. Corchero (UAB)
16:05-16:45 Coffee Break
Session 2 Metabolic Modelling
16:45-17:10 Towards genome-scale metabolic pathway
analysis: metabolome integration allows efficient
enumeration of elementary flux modes in metabolic
networks
J. Zanghellini (ACIB)
17:10-17:35 Metabolic flux analisys in P.pastoris J. Albiol (UAB)
17:35-17:55 Design of optimally constructed metabolic networks
of minimal functionality
D. Ruckerbauer (ACIB)
17:55-18:15 Macrokinetic modelling for comparative heterologous
protein production by Pichia pastoris under AOX1
promoter
F. Valero (UAB)
18:15-18:35 Recombinant Rhamnulose-1-Phosphate Aldolase
production prediction in E.coli fed-batch cultures
J. López (UAB)
18:35- 18:55 The chaperone DnaK controls the conformational
quality of JCV VP1 virus-like particles produced in
Escherichia coli and insect cells
N. Ferrer (UAB)
18:35 Get together + Poster session

3. May, 28th
Session 3 Microbial cell engineering
9:00-9:25 Bacterial inclusion bodies are functional and versatile
biomaterials
A. Villaverde (UAB)
9:25-9:50 Recombinant protein production - rational design of E.
coli expression systems
G. Striedner (ACIB/BOKU)
9:50-10:10 Mass spectrometry based metabolomics - state of the
art and perspectives
S. Hann (ACIB/BOKU)
10:10-10:50 Integrated quantitative metabolomics and metabolic
flux analysis of the physiological response of Pichia
pastoris to recombinant protein production
P. Ferrer (UAB)
10:50-11:15 Coffee Break
11:15-11:40 Systems biotechnology Pichia pastoris: cell design
based on transcriptomics, proteomics and metabolic
modelling
D. Mattanovich
(ACIB/BOKU)
11:40-12:00 New insights from the T. reesei transcriptome to improve
strain engineering towards CAZyme production
R. Bischof (ACIB/TU Vienna)
12:00-12:20 Investigating the physiological effect of increased
heterologous gene dosage in Pichia pastoris using
transcriptomics
E. Cámara (UAB)
12:20-12:40 Methanol induced changes on the transcriptome,
proteome, metabolome and fluxome of Pichia pastoris
H. Rußmayer (ACIB/BOKU)
12:40-14:30 Lunch & Poster Session
Session 4 Biocatalysis and Metabolic engineering
14:30-14:55 Microbial itaconic acid production M. Sauer (ACIB/BOKU)
14:55-15:20 Biocatalysts immobilization and biotransformations G. Álvaro (UAB)
15:20-15:50 Coffee Break
15:50-16:15 (+)-Nootkatone production in yeasts H. Pichler (ACIB/TU Graz)
16:15-16:35 Kinetic resolution of tertiary alcohols by esterase-
variants from strain Bacillus sp. BP-7
A. Fillat (Univ. Barcelona)
16:35-16:55 Microbial adipic acid production H. Marx (BOKU)
16:55-17:00 Closing remarks