Innovation in Rice bran extraction with minimal heat and time

1. by
Health tech for Integrated Health Solutions
CEO Dr. Amr M. Helal
Rice Bran Nutraceuticals
from Waste material
to Products for Healthy aging
Debriefing and Networking
Meeting
RDI – 4.9.2018

2. Before we starts
Numbers Never Lies

3. List of exporters for the selected product in 2013
Product : 1211 Medicinal plants
Value exported in 2013
(USD thousand)
Trade balance in 2013 (USD thousand)
World 3,399,350
China 1,196,725
India 207,758
Canada 184,930
United States of
America
168,286
Germany 148,372
Egypt 109,845
Korea, Republic of 109,379

5. List of exporters for the selected product in 2013
Product : 30 Pharmaceutical products
Trade Indicators
Value exported in 2013 (USD
thousand)
Trade balance in 2013 (USD thousand)
World 486,308,192
Germany 73,779,288
Israel 6,099,722

6. Best Investment !

7. On going Success Story
Supported by EU- RDI/ IMC/ Others
Egyptian Partners
 German University in Cairo
 Faculty of Pharmacy Cairo U.
 National Research center
 Agriculture Research Center
 Health Tech
 Al Sonbolah Rice Mill
 International Trade and
marketing
EU Partners
 Goethe University
 Muenster University
 VIVA cell Biotechnology
 FB- Food Germany
 Analyze and realize (a&r)
 Baspharma Holland
 Holland Food Trade
 Ainia (Spain)

8. Rice Bran
in Egypt
Total Rice cultivated area
 >1.5 Million Acers
 > 500,000 MT Rice bran

9. Rice Milling Processes
Husk removed Bran removed

10. is one of the most nutritive ingredients of the rice crop, where about
60% of the total nutritive value of rice itself resides
Rice BranPROTEIN
OIL DIETARY
FIBRE
VITAMINS &
MINERALS
GAMMA
ORYZANOL
ANTIOXIDANTS
TRACE
ELEMENTS
RICE BRAN
These components can be obtained from other sources in nature
separately, but Rice Bran is unique in having them all

11. Rice Bran Nutraceutical / Functional Food Values
Potent
Antioxidant
High Nutritional Value
Blood
Pressure
Blood
Cholesterol
Potential benefits of RICE BRAN
Blood Sugar

12. However due to non stabilization of Rice
Bran; it is still discarded or used as animal
feed despite its high content of bioactive
substances.
It all starts with IMC support for :
RICE BRAN INNOVATIVE STABILIZATION

13. Project Name Funding Authority Budget
Rice Bran Stabilization Unit (5-7-1)
Faculty of Agriculture, Mansoura
University
2007-2008
Industrial
Modernization
Centre (IMC)
70,000
L.E.
Rice Bran Stabilization Industrial Unit
(1-12-53)
Faculty of Agriculture, Mansoura
University
2009-2010
Industrial
Modernization
Centre (IMC)
700,000
L.E.
Rice Bran Phytochemical Study (3-7-
4)
National Research Centre
2008-2009
Industrial
Modernization
Centre (IMC)
100,000
L.E.
Rice Bran Industry Implementation
(5-7-2)
National Research Centre
Industrial
Modernization
Centre (IMC)
200,000
L.E.

14. Rice Bran Nutraceuticals
• Contract Number MED/2008/214-423
• Total cost of the action EURO 192000 (18 months)
• Project PI: Prof. Dr. M.T. Khyyal
• Project Partners: International trade and Marketing IT&M
(Coordinator), Faculty of Pharmacy; German University in
Cairo GUC; Faculty of Pharmacy; Johann Wolfgang Goethe
Unifersirty Frankfurt (GUF) ; VivaCell Biotechnology GmbH
VC (Germany)

15. • Egyptian rice bran was stabilized immediately after milling to
preserve its active constituents.
• An extract (RBEX) was prepared, analyzed phytochemicaly
and standardized to contain 2.05 % gamma-oryzanol.
• Other active constituents included tocotrienols, tocopherols
and policosanol.
• The extract was used to study the pharmacological
properties outlined below with a view of developing
nutraceutical formulations.
• RBEX was found to have therapeutic potentials in rheumatic
disorders, hypertension, Type 2 diabetes and Alzheimer &
neurodegenerative disorders.
POSTER ABSTRACT

16. Conclusions
Based on present experimental evidence, Egyptian
rice bran extract has therapeutic potentials in:
1. Rheumatic disorders: associated with its anti-
inflammatory, antioxidant activity and effect on
cytokines.
2. Hypertension: related to its antioxidant &
hypocholesterolemic activity.
3. Type 2 Diabetes: effect on insulin release and blood
glucose.
4. Alzheimer and neurodegenerative disorders:
mitochondrial protection.

17. Rice Bran Extract Safety studies

18. -No signs of acute or sub –chronic oral toxicity was observed
over the range of
studied doses (2.0, 1.0 and 0.5 g/kg Rice bran oil extract)
- The doses selected are 20 , 10 and 5 times above the
therapeutic dose.
-Some significant histological and biochemical differences were
observed at the
2.0g/kg rice bran oil extract
-No signs of mutagenicity or genotoxicity was detected for the
rice bran oil extract at gene, DNA or chromosomal level using the
applied techniques in the present study
Conclusions

19. Rice Bran Formulations
• Contract Number: RDI2/S2/26
• Total cost of the action: EURO 104000 (15 months)
• Project PI: Dr. Amr M. Helal
• Project Partners and associates:
– Faculty of Pharmacy Cairo University
– Faculty of Pharmacy; Johann Wolfgang Goethe
University Frankfurt
– Analyze & Realize, Germany
– BasPharma, The Netherlands

20. • The overall objective of the project is
Innovative formulation for stabilized rice bran
extract with maximum therapeutic usefulness
for pharmaceutical, food, beverage and
cosmetic applications
• And the specific objective(s); Stabilized rice
bran prompt extraction at room temperature
and spray drying into instant water - soluble
formulation.

21. Innovation in rice bran extraction technology

22. Innovation in rice bran extraction technology

23. Products under registration
Registered and currently in the market
Stabilized Rice Bran Sachets

24. Products under registration (Cont.)
at the final stage of pricing
(five years procedures
RICPLEX
20 Soft Gel capsules

26. PorridgePlus - a food against dementia
Aim  to develop a food product for elderly with
the objective to promote healthy brain aging

27. Aim of the Project:
• to develop a food product for elderly with the
objective to promote healthy brain aging.
This project primarily based on the findings that Rice
Bran Extract protects from mitochondrial dysfunction
in the brain.
• to develop a rice bran extract as a potential food
supplement to prevent neurodegeneration
“Together with partners from Egypt”

28. Project Outputs Marketing

31. List of Publications
A) Alzheimer’s Disease related
i-Hagl S , Kocher A, Schiborr C, Eckert SH, Ciobanu I, Birringer M, El-
Askary H, Helal A, Khayyal MT, Frank J, Muller WE, Eckert GP. Rice
bran extract protects from mitochondrial dysfunction in guinea pig
brains. Pharmacol Res. 2013; 76: 17-27.
ii-Hagl S, Berressem D, Grewal R, Grebenstein N, Frank J, Eckert GP.
Rice bran extract improves mitochondrial dysfunction in brains of aged
NMRI mice. NutrNeurosci. 2015a Aug 4. [Epub ahead of print]
iii-Hagl S , Berressem D , Bruns B, SusN, Frank J and . Eckert GP.
Beneficial Effects of Ethanolic and Hexanic Rice Bran Extract on
Mitochondrial Function in PC12 Cells and the Search for Bioactive
Components. Molecules. 2015 b; 20(9): 16524-39. doi:
.3390/molecules200916524.
iv- Hagl S, Grewal R, Ciobanu I, Helal A, Khayyal MT, Muller WE,
Eckert GP . Rice Bran Extract compensates mitochondrial
dysfunction in a cellular model of early Alzheimer's disease. J
Alzheimers Dis. 2015c; 43 (3):927-38. doi: 10.3233/JAD-132084.

32. B) Safety and Toxicology
v. Heikal OA, Zickri MB, Helal AM, EL-Askary H, Fiebich L, Gomaa IO.
Stabilized rice bran extract: Acute and 28-day repeated dose oral toxicity with
in vitro mutagenicity and genotoxicity study. African journal of pharmacy and
pharmacology. 2015; 9(44): 1037-1050 DOI: 10.5897/AJPP2014. 4083
C) Pharmacology related
vi. Khayyal M, Abdel-Aziz H, Mohsen R, Helal A, Mueller W, Eckert G.
Pharmacological studies on a standardized n Egyptian rice bran extract . Basic &
clinical pharmacology & toxicology 115, 139-139
vii. Helal AM, Khayyal MT, El Aziz HMA, Salam RMA. Developing a nutraceutical
from Egyptian stabilized rice bran: a pharmacological approach Planta Medica 77
(12), PJ23
viii. Kaup RM, Khayyal MT, Verspohl EJ. Antidiabetic effects of a standardized
Egyptian Rice Bran. Phytotherapy Research . 2013; 27: 264-271 .
C) Pharmaceutical Scineces related
ix-Mohamed Hassan Hany and Omaima Naim El Gazayerly. Rice bran solid lipid
nanoparticles: Preparation and characterization. International Journal of Research
in Drug Delivery. Received 13 August 2011; accepted 08 September 2011.

33. D) Phytochemisty and food sciences
ix. Al-Okbi SY, Hussein AMS, Hamed IM, Mohamed DA, Helal AM. Chemical,
Rheological, Sensorial and Functional Properties of Gelatinized Corn- Rice Bran Flour
Composite Corn Flakes and Tortilla Chips, Journal of Food Processing and Preservation,
2012 (Wiley) , doi:10.1111/j.1745-4549.2012.00747.
x. Ammar HO, Al-Okbi, SY, Mostafa DM, Helal AM. Rice bran oil: Preparation and
evaluation of novel liquisolid and semisolid formulations. International Journal of
Pharmaceutical compounding. 2012; 16: 516-523.
xi. Al-Okbi, SY, Mohamed, DA, Hamed, IM, Agoor, FS, Ramadan, AAM, El-Saed, S, Helal,
AM. Comparative study on Egyptian rice bran extracted by solvents and supercritical CO2.
Advances in Food Sciences. 2013; 35: 23-29.
xii. Al-Okbi, SY, Ammar, NM, Mohamed, DA, Hamed, IM, Desoky, AH, El-Bakry,HF,
Helal, AM. Egyptian rice bran oil: chemical analysis of the main phytochemicals.
Rivista taliana delle Sostanze Grasse 2014 ; 90(1):47-58.
Ahmed M. S. Hussein, Sahar Y. Al-Okbi. Evaluation of Bakery Products Made from Barley-
Gelatinized Corn Flour and Wheat-Defatted Rice Bran Flour Composites.
World Academy of Science, Engineering and Technology -International Journal of
Biological, Biomolecular, Agricultural, Food and Biotechnological Engineering Vol:9,
No:9, 2015

34. INNOVATION AWARDS

35. Sustainability
 Oryza in the market (expected sales in 2019 > 100K
unit.
 Riciplex in the market as from 2019.
 Member in the Phrmacuetical alliance (ASRT)
 Participation within PRIMA (2018), CBC- MED (2018)
(unsuccessful). Innovative product based on rice bran.
- Export to Gulf Countries
- Export to EU (Germany)
- Possible Export to USA

36. Next Phase
 Currently more than 5 PH.D candidates are investigting the
mechanism of action. (Phytochemistry, Pharmacology,
Medicine, Biochemistry)
 Plans for
 Pre clinical studies
 Clinical studies
 Having claims for protection against
dementia/ Alzheimer's disease/ Improve
cognition.

37. Nations’ s Wealth
Is not by what they have
Or what they know
But
By Knowing what to do
with what they have

38. Contact Data
• Address: 118 Mohy El Dein Abo El Ezz. Giza
POC 12411- EGYPT
• Tel: 00202 33045736
• Fax: 00202 33477755
• Email: dr.amrhelal@healhtech-eg.com
• Web site : www.healthtech-eg.com