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NUTRIENT AGAR
• Nacl : 0.5gm
• Agar : 2.0gm
• D/w : 100ml
• ph : 7.2 – 7.5
Potato infusion form : 20.0gm
Dextrose : 2.0gm
Starch : 0.1gm
Agar : 2.0gm
D/w : 100ml
Ph : 5.6
Sucrose : 3.0gm
NaNo3 : 0.2gm
K2HPo4 : 0.1gm
MgSo4.7H2O : 0.05gm
Kcl : 0.05gm
FeSo4 : 0.001gm
Bromothymol blue : 4.0ml (1% w/v)
Agar : 2.0gm
D/w : 100ml
ph : 6.0
BLOOD AGAR MEDIA
Peptone : 1.0gm
Beef extract : 1.0gm
Nacl : 0.5gm
Agar : 2.0gm
D/w : 100ml
Blood : 10ml
ph : 7.2
Protease peptone : 0.5gm
Nacl : 0.5gm
Sodium hydrogen
phosphate (Na2po4) : 0.5gm
Dextrose : 0.05gm
Agar : 2.0gm
D/w : 100ml
ph : 7.2
Mannitol : 1.0gm
Peptone : 1.0gm
Nacl : 0.45gm
Beef extract : 0.1gm
Phenol red : 0.0025gm
Agar : 2.0gm
D/w : 100ml
ph : 7.4
Peptone : 2.0gm
Nacl : 0.5gm
Bile salt : 1.5gm
Lactose : 1.0gm
Neutral red solution
(1% aqu) : 1.0ml
Agar : 2.0gm
D/w : 100ml
ph : 7.1
Peptone : 1.0gm
Lactose : 0.5gm
Potassium dihydrogen
phosphate (k2h2po4) : 0.2gm
Eosin yellow : 0.04gm
Methylene blue : 0.0065gm
Agar : 2.0gm
D/w : 100ml
ph : 7.2
Soluble starch : 2.0gm
Peptone : 0.5gm
Beef extract : 0.3gm
Agar : 2.0gm
D/w : 100ml
ph : 7.2
Yeast extract : 0.45gm
Peptone : 0.75gm
Dextrose : 2.0gm
Agar : 2.0gm
D/w : 100ml
ph : 6.5 – 7.0

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Medias folder

  • 1. NUTRIENT AGAR • Nacl : 0.5gm • Agar : 2.0gm • D/w : 100ml • ph : 7.2 – 7.5
  • 2. Potato infusion form : 20.0gm Dextrose : 2.0gm Starch : 0.1gm Agar : 2.0gm D/w : 100ml Ph : 5.6
  • 3. Sucrose : 3.0gm NaNo3 : 0.2gm K2HPo4 : 0.1gm MgSo4.7H2O : 0.05gm Kcl : 0.05gm FeSo4 : 0.001gm Bromothymol blue : 4.0ml (1% w/v) Agar : 2.0gm D/w : 100ml ph : 6.0
  • 4. BLOOD AGAR MEDIA Peptone : 1.0gm Beef extract : 1.0gm Nacl : 0.5gm Agar : 2.0gm D/w : 100ml Blood : 10ml ph : 7.2
  • 5. Protease peptone : 0.5gm Nacl : 0.5gm Sodium hydrogen phosphate (Na2po4) : 0.5gm Dextrose : 0.05gm Agar : 2.0gm D/w : 100ml ph : 7.2
  • 6. Mannitol : 1.0gm Peptone : 1.0gm Nacl : 0.45gm Beef extract : 0.1gm Phenol red : 0.0025gm Agar : 2.0gm D/w : 100ml ph : 7.4
  • 7. Peptone : 2.0gm Nacl : 0.5gm Bile salt : 1.5gm Lactose : 1.0gm Neutral red solution (1% aqu) : 1.0ml Agar : 2.0gm D/w : 100ml ph : 7.1
  • 8. Peptone : 1.0gm Lactose : 0.5gm Potassium dihydrogen phosphate (k2h2po4) : 0.2gm Eosin yellow : 0.04gm Methylene blue : 0.0065gm Agar : 2.0gm D/w : 100ml ph : 7.2
  • 9. Soluble starch : 2.0gm Peptone : 0.5gm Beef extract : 0.3gm Agar : 2.0gm D/w : 100ml ph : 7.2
  • 10. Yeast extract : 0.45gm Peptone : 0.75gm Dextrose : 2.0gm Agar : 2.0gm D/w : 100ml ph : 6.5 – 7.0