Laboratory of Cancerology and Experimental Toxicology

1. Toxicological Risk Assessment
An initiative of
24 October 2013 - UCL - Brussels (Woluwe)
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2. Laboratory of Cancerology and Experimental
Toxicology - Faculty of Pharmacy
Université Libre de Bruxelles
Pr Véronique Mathieu
MD, Ph.D.
Associate Professor
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3. Fields of expertise and industrial targets
Fields of expertise
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Natural (derived) compounds
Apoptosis-resistance
migration- invasion
Anti-cancer activities
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Galectin-1
Actin
Gene silencing: siRNA
Videomiscroscopy
Industrial applications fields
• Discovery of new chemical entities to combat apoptotic
resistant cancers like glioma, melanoma, non-small cell lung
carcinoma….
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4. Applied research projects:
example of galectin-1
Pub. No.:
WO/2006/108474
Publication
Date:
19.10.2006
Chapter 2 Demand Filed: 09.02.2007
International Application No.: PCT/EP2006/00217
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International Filing Date:
09.03.2006
IPC:
C12N 15/11 (2006.01), A61K 48/00 (2006.01)
Applicants: UNIVERSITE LIBRE DE BRUXELLES [BE/BE]; Avenue F.D. Roosevelt 50, CP 161, B-1050 Brussels (BE) (All Excep
UNIVERSITE CATHOLIQUE DE LOUVAIN [BE/BE]; Place De L'université 1, B-1348 Louvain-la-Neuve (BE) (All Exce
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CAMBY, Isabelle [BE/BE]; Rue Ferrer 22, B-1480 Tubize (BE) (US Only).
HENRIET, Patrick [BE/BE]; Rue De La Briqueterie 17, B-1400 Nivelles (BE) (US Only).

5. Special equipments
Examples
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Culture rooms
Flow cytometry (3 colors)
Fluorescent microscope
ELISA
(RT)-PCR, qPCR
WB
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16 videomicoscopic devices
Quantitative videomicroscopy > 20 years of expertise
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• Animal facilities and cancer aggressive models
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6. Computer-Assisted Phase-Contrast Microscopy
(Quantitative Videomicroscopy)
SKMEL28 Melanoma Cells / Control
Debeir O et al. IEEE Trans Med Imaging 2005
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7. The cells are cultured for 3 days in presence of the compound
x 250
Dumont P et al., Neoplasia 2007
Anti-adhesive ?
Computer assisted videomicroscopy
Increasing differentiation
Soft agar test
Cytostatic ?
MTT colorimetric test
Increasing proliferation potentiel
Cytotoxic ?

8. Services provided to companies
Examples
• MTT assay on normal and cancer cells
• Quantitative videomicroscopy experiment
• Cell cycle and / or apoptosis analysis
• ROS production analysis
• Migration assays (boyden chambers, quantitative scratch
wound assay)
• Actin or tubulin cytoskeleton analysis (fluorescence)
• Mechanism of action deciphering (adapted to your project)
• Maximal tolerated dose in vivo in conventional mice
• Anti-cancer activity on cancer bearing mice
• …
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9. Training proposals to companies
• PhD training
• Post-Doctoral fellowship
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10. Contacts
Université Libre de Bruxelles - Faculty of Pharmacy
Laboratory of Cancerology and Experimental Toxicology
Boulevard du Triomphe
Campus de la Plaine, CP205/1
Building BC
1050 Brussels
http://www.ulb.ac.be/rech/inventaire/unites/ULB646.html
Pr Véronique Mathieu
Associate Professor
vemathie@ulb.ac.be
 +32 478 317 388
Pr Jean-Michel Kauffmann
Head of the Laboratory
jmkauf@ulb.ac.be
 +32 2 650 52 15
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