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IJRET: International Journal of Research in Engineering and Technology eISSN: 2319-1163 | pISSN: 2321-7308
_______________________________________________________________________________________
Volume: 04 Issue: 03 | Mar-2015, Available @ http://www.ijret.org 263
IMAGE SEGMENTATION METHODS FOR BRAIN MRI IMAGES
Swapnali Matkar1
, Megha Borse2
1
Dept. of Electronics &Telecommunication, Cummins College of Engineering for women, Maharashtra, India
2
Assistant Professor, Dept. of Electronics &Telecommunication, Cummins College of Engineering for Women,
Maharashtra, India
Abstract
In Image Processing, extracting the region of interest is a very challenging task. To extract information, pre-processing
algorithms are important in MRI image. Edge detection is a task in which points in image are identified at which brightness
changes sharply or it has discontinuities. It is an essential pre- processing step in medical image segmentation, for object
recognition of the human organs. The applications of medical image segmentation are 3D reconstruction and quantitative analysis
and so on. We used MRI images because MRI images give best view of tissues in any part of human body. In this paper, difficulties
of edge detection in brain magnetic resonance images are considered and a new approach to edge detection is introduced. There
are many traditional edge detection methods for extracting edges from images have been introduced such as gradient based
operators like sobel, prewitt, robert were initially used for edge detection, but they did not give sharp edges and were highly
sensitive to noise image. And in medical field accuracy is important fact. To overcome these difficulties, we proposed new method
called as Active Contour method or snake model. . In the field of medical segmentation, Active contour method is one of popular
research topic. This method is used for detecting brain region based on their energy function. In order to compare between them,
one slice of MRI image tested with these methods. The traditional and proposed edge detection algorithms are implemented in
MATLAB and results of proposed method are presented and compared with traditional approach.
Keywords: Edge detection, Brain MRI images, Canny edge detector, Active contour method and MATLAB.
-------------------------------------------------------------------***--------------------------------------------------------------------
1. INTRODUCTION
Medical imaging is a significant application of image
processing for clinical purposes such as diagnose, examine
disease or for medical research. There are various types of
imaging techniques are used to study brain anatomy and
functions like CT scan, MRI, PET etc. But MRI images are
widely used because they provide best visualization of
abnormalities of brain also they provide high quality images
with good soft tissue contrast.
The basic feature of image is Edge. In the image processing,
edge detection is a very critical function since edge contains
major information about image. The basic function of edge
detection is to protect the important structural properties of
image and filter out the useless information to reduce the
amount of data. There are many edge detection methods
exist for medical images. In this paper we make comparison
between some of traditional methods and new approach for
edge detection i.e. Active Contour Method.
In the first section we discussed different edge detection
algorithms like robert, Prewitt, Sobel are Gradient based
classical operators initially used for edge detection. These
methods are used to find out approximate absolute gradient
magnitude at each point in an input grayscale image but they
did not give sharp edges and were highly sensitive to noise
image. Canny edge detector uses Gaussian filter to de-
noised the image and then apply non-maximum suppression.
In next section we discussed about the concept of active
contours which was introduced by Kass. The two properties
of Active contour i.e. smoothness and continuity, capture
edge of curved object sharply. In the last section
experimental results are shown and comparison is done
between these methods.
2. TRADITIONAL EDGE DETECTION
METHODS
We begin by introducing some of traditional approaches for
edge detection.
1. Sobel Edge Detector
2. Prewitt Edge Detector
3. Canny Edge Detector
2.1. Sobel Edge Detection [5]
Sobel Edge detection method is very simple to implement.
We have to compute the partial derivatives at every pixel
location in the image to obtain the gradient of an image.
These derivatives can be derived by using two 3x3 size
masks. The mask is slide over image, one estimating the
gradient in the x-direction and the other estimating the
gradient in the y-direction.
IJRET: International Journal of Research in Engineering and Technology eISSN: 2319-1163 | pISSN: 2321-7308
_______________________________________________________________________________________
Volume: 04 Issue: 03 | Mar-2015, Available @ http://www.ijret.org 264
Fig -1: Sobel convolution mask
We move the sobel mask over the MRI image to calculate
horizontal and vertical gradient (Gx and Gy) ,then we
combine it together to find magnitude and orientation of
point. Magnitude gives us strength and orientation gives us
direction of pixel
Magnitude=√(Gx2
+Gy2
) (1)
Direction=tan1
(Gy/Gx) (2)
2.2 Prewitt Edge Detection [5]
The implementation of prewitt edge detector is same as
sobel edge detector but have mask of different coefficient.
Both masks are convolved with the image and summed to
get output.
Fig -2: Prewitt convolution mask
2.3 Canny Edge Detection [1]
Canny Edge detector is optimal edge detector. It works in
multi-stage process. The algorithm of canny detector has
following steps:
1. Smoothing using Gaussian Filter
2. Calculate gradient magnitude
3. Non maxima suppression
4. Thresholding
In order to remove the noise, filter the image is very
essential step. Gaussian filter is convolved with image to
obtain smooth image. Then by using sobel or prewitt
operator, we find gradient magnitude and orientation. After
calculating gradient magnitude we get blurred edges in the
image. To convert the blurred edges into sharp edges we
have done non maxima suppression. In non- maxima
suppression, edge strength of current pixel is compare with
pixel in +ve and –ve direction. If current pixel magnitude is
larger than other pixels, keep the value of the magnitude
otherwise suppress the value. To detect and link the edges,
we use double threshold algorithm. If we use only low
threshold, noisy maxima will get added and if we use high
threshold, there is a possibility of true maxima might be
missed. Therefore in canny method double threshold is used.
The strong edge points than high threshold are set as strong
and points weaker than low threshold are suppressed and
points between thresholds are set as weak.
Fig -3: Comparision between sobel,prewitt and canny
detector
3. PROPOSED EDGE DETECTION METHOD [2]
The difference in grey level values in the medical images are
not very clear, therefore the edges of organs are always
vague. Often edge obtain after canny detector is not
continuous, to improve segmentation, Active contour
method have been widely used in medical image analysis. It
is also called as snake model. The snake model depends on
the energy function of curve. Snake model is a parametric
curve which is under influence of internal and external
energies. Where energy function of edge is minimum, curve
tries to converge in that direction.
Basic Principle:
The basic contour curve is defined as:
V(s)=(x(s).y(s)) (3)
Energy function is given as :
E_model= E_int+E_ext(includesE_img) (4)
IJRET: International Journal of Research in Engineering and Technology eISSN: 2319-1163 | pISSN: 2321-7308
_______________________________________________________________________________________
Volume: 04 Issue: 03 | Mar-2015, Available @ http://www.ijret.org 265
There are basically three energies functions:
1. Internal energy function
2. External energy function
3. Image energy function
By using Eint, the internal energy we can control the shape
and smoothness of contour. Eint can be written as:
E_int= ½ [∫α|v’(s)|
2
+β|v”(s)|2
]ds (5)
Internal energy gives smoothness and curvature. In the
above equation, the measure of ealsticity has been given by
first order term of contour curve. The 2nd
oeder term gives
measure of curvature. The elasticity value is high where
curve get streched and the curvature value is high where
curve get kinked. Α and β are continuity and degree of
bending paraments respectivily. We can get more
deformation by lowering these parameters.
Eext, the external energy itself includes the image energy. The
external energy helps to guide the behaviour of
segmentation. And the image energy helps for durecting the
deformation of contour, It pushes the contour model towards
the position of object edge. The Steps for Active contour
model are given as follows: [3]
1. First we set the initial contour around the object
boundary. This initial contour should be close to the
boundary to get the close deformation.
2. Plant the searching lines. And on each searching create
candidate points.
3. Calculate the energy for each candidate point using
energy function formula.
4. Select the suitable points with minimum energy.
Minimum energy shows better possibilities to be close to
object contour.
5. Connect that best matched points to form closed contour.
From above procedure segmentation is done completely.
4. EXPERIMENT
To perform different edge detection methods we used brain
MRI image of type T1 weighted. The size of image is
131x131. First convert the image to grayscale image before
applying the edge detection algorithm. All the operations are
performed in MATLAB. The output of sobel, prewitt and
canny edge detector, we can see in figure 3. The sobel and
prewitt operator did not show detail information because
most of the data is lost. For the further study in medical
image analysis required detail information which cannot
produce by using these edge detector. Canny edge detector
can produce output with better localization. Also it is less
sensitive to noise unlike sobel and prewitt edge detector. It
works fine under the noisy condition. But if we set threshold
value too high, then we can loss some important
information. And if threshold value is too low, then some
unwanted information might be get added. So giving
threshold value which works well on all images is very
difficult.
Active contour model has interactive ability and overcomes
shortcomings of traditional methods.This method gives
better output, once an appropriate initialization is done. The
brain image is more concave. By using this method this
concave region can be processed in better way. [4]
Fig -4: Initial contour around object boundary
Fig -5: Final Segmented Imag
These traditional methods were compared with active
contour method using two different criteria.
1) Visual quality comparison 2) PSNR value.
A larger PSNR value indicates better quality of image. After
analyzing the PSNR of the test cases, it is clear that the
active contour method performed well on the image.
Table 1 compares PSNR values of these methods.
Edge Detection Method PSNR Value
Sobel Edge Detection 13.9112
Prewitt Edge Detection 13.9107
Canny Edge Detection 13.9222
Active Contour 43.2072
IJRET: International Journal of Research in Engineering and Technology eISSN: 2319-1163 | pISSN: 2321-7308
_______________________________________________________________________________________
Volume: 04 Issue: 03 | Mar-2015, Available @ http://www.ijret.org 266
5. CONCLUSION
In the medical image processing accuracy is the important
factor. The output of sobel and prewitt edge detector is not
enough accurate with the thin and smooth edges. In canny
edge detector, sometimes edge is spurious and they contain
holes along curve which is not desirable. Also in the
traditional methods, we convolve image with horizontal and
vertical gradient operators which gives detection of edge in
favour of rectilinear object only. In the field of medical
segmentation, Active contour method is one of popular
research topic. Two important properties of Active contour
such as smoothness and continuity increase the accuracy of
segmentation. It is more suitable to capture curved object
boundaries. In comparison with traditional method of edge
detection, Active contour method gives better segmentation
and accuracy.
REFERENCES
[1]. Zolqernine Othman, Habibollah Haron, Mohammed
Rafiq Abdul Kadir, "Comparison of Canny and Sobel Edge
Detection in MRI Images", University Teknologi
Malaysia,Pg.no. 133-134.
[2]. Jinyong Cheng, Yihui Liu, Ruixiang Jia, Weiyu Guo,
"A New Active Contour Model for Medical Image Analysis
Wavelet Vector Flow", IAENG International Journal of
Applied Mathematics, Volume 36, Pg.no. ,429-430,May
2007.
[3]. Jinyong Cheng, Yihui Liu, "3-D Reconstruction of
Medical Image Using Wavelet Transform and Snake
Model", JOURNAL OF MULTIMEDIA, VOL. 4,Pg.no-
428-430,(2009).
[4]. Jianhua Liu, Jianwei Wang, "Application of Snake
Model in Medical Image Segmentation", Journal of
Convergence Information Technology(JCIT), Vol.
9,Pg.no-105-106,2004.
[5]. R.C.Gonzalez and R.E.Woods, "Digital Image
Processing",Addison-Wesley,Reading,Pg.no.744 ,(1992).
BIOGRAPHIES
Ms.Swapnali Matkar is pursuing M. E.
degree in Signal Processing from
Cummins College of Engineering for
women under Pune University. She is
received B.E degree in Instrumentation
from Mumbai University
Mrs..Megha Sunil Borse received
M.E.degree in Electronics Digital
Systems from Pune University. She is
registered PhD student of Pacific
Uniersity, Udaipur. She is currently
working as a Assistant Professor in
Cummins College of Engineering for women, Pune. She has
total 16 years of teaching experience. Her area of
specialization is biomedical image processing.

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Image segmentation methods for brain mri images

  • 1. IJRET: International Journal of Research in Engineering and Technology eISSN: 2319-1163 | pISSN: 2321-7308 _______________________________________________________________________________________ Volume: 04 Issue: 03 | Mar-2015, Available @ http://www.ijret.org 263 IMAGE SEGMENTATION METHODS FOR BRAIN MRI IMAGES Swapnali Matkar1 , Megha Borse2 1 Dept. of Electronics &Telecommunication, Cummins College of Engineering for women, Maharashtra, India 2 Assistant Professor, Dept. of Electronics &Telecommunication, Cummins College of Engineering for Women, Maharashtra, India Abstract In Image Processing, extracting the region of interest is a very challenging task. To extract information, pre-processing algorithms are important in MRI image. Edge detection is a task in which points in image are identified at which brightness changes sharply or it has discontinuities. It is an essential pre- processing step in medical image segmentation, for object recognition of the human organs. The applications of medical image segmentation are 3D reconstruction and quantitative analysis and so on. We used MRI images because MRI images give best view of tissues in any part of human body. In this paper, difficulties of edge detection in brain magnetic resonance images are considered and a new approach to edge detection is introduced. There are many traditional edge detection methods for extracting edges from images have been introduced such as gradient based operators like sobel, prewitt, robert were initially used for edge detection, but they did not give sharp edges and were highly sensitive to noise image. And in medical field accuracy is important fact. To overcome these difficulties, we proposed new method called as Active Contour method or snake model. . In the field of medical segmentation, Active contour method is one of popular research topic. This method is used for detecting brain region based on their energy function. In order to compare between them, one slice of MRI image tested with these methods. The traditional and proposed edge detection algorithms are implemented in MATLAB and results of proposed method are presented and compared with traditional approach. Keywords: Edge detection, Brain MRI images, Canny edge detector, Active contour method and MATLAB. -------------------------------------------------------------------***-------------------------------------------------------------------- 1. INTRODUCTION Medical imaging is a significant application of image processing for clinical purposes such as diagnose, examine disease or for medical research. There are various types of imaging techniques are used to study brain anatomy and functions like CT scan, MRI, PET etc. But MRI images are widely used because they provide best visualization of abnormalities of brain also they provide high quality images with good soft tissue contrast. The basic feature of image is Edge. In the image processing, edge detection is a very critical function since edge contains major information about image. The basic function of edge detection is to protect the important structural properties of image and filter out the useless information to reduce the amount of data. There are many edge detection methods exist for medical images. In this paper we make comparison between some of traditional methods and new approach for edge detection i.e. Active Contour Method. In the first section we discussed different edge detection algorithms like robert, Prewitt, Sobel are Gradient based classical operators initially used for edge detection. These methods are used to find out approximate absolute gradient magnitude at each point in an input grayscale image but they did not give sharp edges and were highly sensitive to noise image. Canny edge detector uses Gaussian filter to de- noised the image and then apply non-maximum suppression. In next section we discussed about the concept of active contours which was introduced by Kass. The two properties of Active contour i.e. smoothness and continuity, capture edge of curved object sharply. In the last section experimental results are shown and comparison is done between these methods. 2. TRADITIONAL EDGE DETECTION METHODS We begin by introducing some of traditional approaches for edge detection. 1. Sobel Edge Detector 2. Prewitt Edge Detector 3. Canny Edge Detector 2.1. Sobel Edge Detection [5] Sobel Edge detection method is very simple to implement. We have to compute the partial derivatives at every pixel location in the image to obtain the gradient of an image. These derivatives can be derived by using two 3x3 size masks. The mask is slide over image, one estimating the gradient in the x-direction and the other estimating the gradient in the y-direction.
  • 2. IJRET: International Journal of Research in Engineering and Technology eISSN: 2319-1163 | pISSN: 2321-7308 _______________________________________________________________________________________ Volume: 04 Issue: 03 | Mar-2015, Available @ http://www.ijret.org 264 Fig -1: Sobel convolution mask We move the sobel mask over the MRI image to calculate horizontal and vertical gradient (Gx and Gy) ,then we combine it together to find magnitude and orientation of point. Magnitude gives us strength and orientation gives us direction of pixel Magnitude=√(Gx2 +Gy2 ) (1) Direction=tan1 (Gy/Gx) (2) 2.2 Prewitt Edge Detection [5] The implementation of prewitt edge detector is same as sobel edge detector but have mask of different coefficient. Both masks are convolved with the image and summed to get output. Fig -2: Prewitt convolution mask 2.3 Canny Edge Detection [1] Canny Edge detector is optimal edge detector. It works in multi-stage process. The algorithm of canny detector has following steps: 1. Smoothing using Gaussian Filter 2. Calculate gradient magnitude 3. Non maxima suppression 4. Thresholding In order to remove the noise, filter the image is very essential step. Gaussian filter is convolved with image to obtain smooth image. Then by using sobel or prewitt operator, we find gradient magnitude and orientation. After calculating gradient magnitude we get blurred edges in the image. To convert the blurred edges into sharp edges we have done non maxima suppression. In non- maxima suppression, edge strength of current pixel is compare with pixel in +ve and –ve direction. If current pixel magnitude is larger than other pixels, keep the value of the magnitude otherwise suppress the value. To detect and link the edges, we use double threshold algorithm. If we use only low threshold, noisy maxima will get added and if we use high threshold, there is a possibility of true maxima might be missed. Therefore in canny method double threshold is used. The strong edge points than high threshold are set as strong and points weaker than low threshold are suppressed and points between thresholds are set as weak. Fig -3: Comparision between sobel,prewitt and canny detector 3. PROPOSED EDGE DETECTION METHOD [2] The difference in grey level values in the medical images are not very clear, therefore the edges of organs are always vague. Often edge obtain after canny detector is not continuous, to improve segmentation, Active contour method have been widely used in medical image analysis. It is also called as snake model. The snake model depends on the energy function of curve. Snake model is a parametric curve which is under influence of internal and external energies. Where energy function of edge is minimum, curve tries to converge in that direction. Basic Principle: The basic contour curve is defined as: V(s)=(x(s).y(s)) (3) Energy function is given as : E_model= E_int+E_ext(includesE_img) (4)
  • 3. IJRET: International Journal of Research in Engineering and Technology eISSN: 2319-1163 | pISSN: 2321-7308 _______________________________________________________________________________________ Volume: 04 Issue: 03 | Mar-2015, Available @ http://www.ijret.org 265 There are basically three energies functions: 1. Internal energy function 2. External energy function 3. Image energy function By using Eint, the internal energy we can control the shape and smoothness of contour. Eint can be written as: E_int= ½ [∫α|v’(s)| 2 +β|v”(s)|2 ]ds (5) Internal energy gives smoothness and curvature. In the above equation, the measure of ealsticity has been given by first order term of contour curve. The 2nd oeder term gives measure of curvature. The elasticity value is high where curve get streched and the curvature value is high where curve get kinked. Α and β are continuity and degree of bending paraments respectivily. We can get more deformation by lowering these parameters. Eext, the external energy itself includes the image energy. The external energy helps to guide the behaviour of segmentation. And the image energy helps for durecting the deformation of contour, It pushes the contour model towards the position of object edge. The Steps for Active contour model are given as follows: [3] 1. First we set the initial contour around the object boundary. This initial contour should be close to the boundary to get the close deformation. 2. Plant the searching lines. And on each searching create candidate points. 3. Calculate the energy for each candidate point using energy function formula. 4. Select the suitable points with minimum energy. Minimum energy shows better possibilities to be close to object contour. 5. Connect that best matched points to form closed contour. From above procedure segmentation is done completely. 4. EXPERIMENT To perform different edge detection methods we used brain MRI image of type T1 weighted. The size of image is 131x131. First convert the image to grayscale image before applying the edge detection algorithm. All the operations are performed in MATLAB. The output of sobel, prewitt and canny edge detector, we can see in figure 3. The sobel and prewitt operator did not show detail information because most of the data is lost. For the further study in medical image analysis required detail information which cannot produce by using these edge detector. Canny edge detector can produce output with better localization. Also it is less sensitive to noise unlike sobel and prewitt edge detector. It works fine under the noisy condition. But if we set threshold value too high, then we can loss some important information. And if threshold value is too low, then some unwanted information might be get added. So giving threshold value which works well on all images is very difficult. Active contour model has interactive ability and overcomes shortcomings of traditional methods.This method gives better output, once an appropriate initialization is done. The brain image is more concave. By using this method this concave region can be processed in better way. [4] Fig -4: Initial contour around object boundary Fig -5: Final Segmented Imag These traditional methods were compared with active contour method using two different criteria. 1) Visual quality comparison 2) PSNR value. A larger PSNR value indicates better quality of image. After analyzing the PSNR of the test cases, it is clear that the active contour method performed well on the image. Table 1 compares PSNR values of these methods. Edge Detection Method PSNR Value Sobel Edge Detection 13.9112 Prewitt Edge Detection 13.9107 Canny Edge Detection 13.9222 Active Contour 43.2072
  • 4. IJRET: International Journal of Research in Engineering and Technology eISSN: 2319-1163 | pISSN: 2321-7308 _______________________________________________________________________________________ Volume: 04 Issue: 03 | Mar-2015, Available @ http://www.ijret.org 266 5. CONCLUSION In the medical image processing accuracy is the important factor. The output of sobel and prewitt edge detector is not enough accurate with the thin and smooth edges. In canny edge detector, sometimes edge is spurious and they contain holes along curve which is not desirable. Also in the traditional methods, we convolve image with horizontal and vertical gradient operators which gives detection of edge in favour of rectilinear object only. In the field of medical segmentation, Active contour method is one of popular research topic. Two important properties of Active contour such as smoothness and continuity increase the accuracy of segmentation. It is more suitable to capture curved object boundaries. In comparison with traditional method of edge detection, Active contour method gives better segmentation and accuracy. REFERENCES [1]. Zolqernine Othman, Habibollah Haron, Mohammed Rafiq Abdul Kadir, "Comparison of Canny and Sobel Edge Detection in MRI Images", University Teknologi Malaysia,Pg.no. 133-134. [2]. Jinyong Cheng, Yihui Liu, Ruixiang Jia, Weiyu Guo, "A New Active Contour Model for Medical Image Analysis Wavelet Vector Flow", IAENG International Journal of Applied Mathematics, Volume 36, Pg.no. ,429-430,May 2007. [3]. Jinyong Cheng, Yihui Liu, "3-D Reconstruction of Medical Image Using Wavelet Transform and Snake Model", JOURNAL OF MULTIMEDIA, VOL. 4,Pg.no- 428-430,(2009). [4]. Jianhua Liu, Jianwei Wang, "Application of Snake Model in Medical Image Segmentation", Journal of Convergence Information Technology(JCIT), Vol. 9,Pg.no-105-106,2004. [5]. R.C.Gonzalez and R.E.Woods, "Digital Image Processing",Addison-Wesley,Reading,Pg.no.744 ,(1992). BIOGRAPHIES Ms.Swapnali Matkar is pursuing M. E. degree in Signal Processing from Cummins College of Engineering for women under Pune University. She is received B.E degree in Instrumentation from Mumbai University Mrs..Megha Sunil Borse received M.E.degree in Electronics Digital Systems from Pune University. She is registered PhD student of Pacific Uniersity, Udaipur. She is currently working as a Assistant Professor in Cummins College of Engineering for women, Pune. She has total 16 years of teaching experience. Her area of specialization is biomedical image processing.