8. Dream No.1 Flow cytometry can be used for clinical leukaemia diagnosis on the ‘immunophenotyping’ platform anti-ALL (24 absorptions!) fetal development: BM is a lymphoid organ Melvyn Greaves, Geoff Brown and George Janossy
9. Dream No.2 from G.Janossy Monoclonal antibodies can be used for visualizing cells in tissues, normal and pathological (‘monoclonal immunohistology’ platform) CD1 (NA1/34) on human thymus: The first ever tissue section stained by the first anti-human monoclonal antibody CD1 (NA1-34) and CD45 (2D1) for T-ALL diagnosis and thymic origin Ken Bradstock, Gianni Pizzolo Bradstock KF, Janossy G, Bollum FJ, Milstein C. Anomalous phenotype in thymic acute lymphoblastic leukaemia. Nature 1980; 284(5755): 455-7.
10. Anticorps monoclonaux Lignée de myélome murin Déficiente en HGPRT Immunisation Rate Pas de fusion - - + Sélection des clones réactifs Clones Fusion en milieu HAT HYPOXANTHINE AMINOPTERINE THYMIDINE
25. ORGANISATION DES FEUILLETS BETA-PLISSES Les feuillets clairs sont sur le dessus (cercle clair), les feuillets foncés en-dessous (cercle foncé) Ils sont reliés entre eux par des boucles dont certaines peuvent porter des CDR PONT DISULFURE Représentation schématique de face Représentation schématique de profil
47. Beta 1 = CD29 Alpha 1 à 6 = VLA 1 à 7 = CD49 a à g Beta 2 = CD18 Alpha L = CD11a Alpha M = CD11b Alpha X = CD11c Alpha E = CD103… RGD receptors = Tripeptide Arg-Gly-Asp
48.
49.
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59.
60.
61. Marqueurs associés à la lignée B CD19 ITAM CD22 or ITIM ITIM ITIM ITIM ITAM CD20 P CD24 CD21 BCR NH2 COOH CD23
63. Marqueurs associés aux cellules myéloïdes CD13 E NH2 COOH E CD14 L L L L L L L L L L P CD33 CD11b Mg Mg Mg MPO K CD117 CD15 CD65 3-fucosyl-N-acetyl-lactosamine céramide dodecasaccharide CD36 CD35 CD16
65. http://1degreebio.org/ De la NLM S Shaw PROW … 339 http://www.sciencegateway.org/resources/prow/index.html à Via l’ASSIM … 363 http://www.thefcn.com/cd_list_hlda_9_barcelona_2010.html
66.
Notes de l'éditeur
The membrane microdomains include lipid rafts, tetraspanin-enriched microdomains (TEM) and podosomes or invadopodia. The association on the tumour cell membrane of cathepsin B with caveolae, a subset of lipid rafts that contain the structural protein caveolin, is mediated by a direct interaction of procathepsin B with the light chain of the annexin II heterotetramer (AIIT). Membrane microdomains are dynamic with constituents also present in intracellular vesicles such as multivesicular bodies, MHC (major histocompatibility complex) class II compartments (MIIC) and exosomes that also contain cysteine cathepsins such as (pro) cathepsin L and cathepsin S. These associations might enhance secretion of cysteine cathepsins, including at podosomes or invadopodia, at which sites CD63 co-localizes with p61Hck, a 'lysosome-associated' Src family kinase. The urokinase plasminogen activator receptor in association with 1-integrin is found in many of the same membrane microdomains, perhaps indicative of an association between these two proteolytic pathways at this sites.